Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152726_jwkn CCCCTGTCCTGCTGATGCTGTCTATCCCCTGG(+AAAAAA)GATCCAGGGTCCTCTGCCTGCCCTCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240606152723_8w4x CTTTTCCAAAGGAGGGCAGGCAGAGGACCCTG(+AAAAAA)GATCCCAGGGGATAGACAGCATCAGCAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606152722_ej3a TTCCTGACGGCTGATGTGGGCCAGTGGCAGAG(+AAAAAA)GATCCAGGTTGCCCTTCCTTCCCCCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240606152720_gnxi TTCCTGTGGTGGGGGGAAGGAAGGGCAACCTG(+AAAAAA)GATCCTCTGCCACTGGCCCACATCAGCCGTC NGG 10 8 17 8 24 0 100 5 10
    240606152718_969p TGGGACACTTGGCAGAAGGGATTGCCCTGGGG(+AAAAAA)GTACCAAGAGCCTAGGGTGCTCCCTGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606152717_xyfk CTGGGACCTACAGGGAGCACCCTAGGCTCTTG(+AAAAAA)GTACCCCCAGGGCAATCCCTTCTGCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606152715_i5hh CCTGCGTCTCCCAGCATGGTAGGTAGCCTCAG(+AAAAAA)GATCCTAGAAAGATTATGGCCAGTGTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240606152714_1erd GTCAGAACAGACACTGGCCATAATCTTTCTAG(+AAAAAA)GATCCTGAGGCTACCTACCATGCTGGGAGAC NGG 10 8 17 8 24 0 100 5 10
    240606152712_ej3w CAAGCAACTGGTAGCTCTCACAATTCTGTTGG(+AAAAAA)GATCCTTTGTGCAATATTGTAGCCATCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606152711_2jx6 CCAGGGGAAGATGGCTACAATATTGCACAAAG(+AAAAAA)GATCCCAACAGAATTGTGAGAGCTACCAGTT NGG 10 8 17 8 24 0 100 5 10