Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152918_7wd4 CACCAGGCTAGGCTCATGGCCTTGAACTCAGG(+AAAAAA)GATCCACCTGCCTCTGCCTCCCTGTGATGCT NGG 10 8 17 8 24 0 100 5 10
    240606152916_1ccp ATCTCAGCATCACAGGGAGGCAGAGGCAGGTG(+AAAAAA)GATCCCTGAGTTCAAGGCCATGAGCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240606152915_oq3u GCCTGAAGAGGGCCTGGGGAACAGTGGAAATG(+AAAAAA)GTACCTTCATGGTGACTTCAGCAGGGTAGTC NGG 10 8 17 8 24 0 100 5 10
    240606152914_wmx6 GTAATGACTACCCTGCTGAAGTCACCATGAAG(+AAAAAA)GTACCATTTCCACTGTTCCCCAGGCCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606152913_l3fc ATAGCTGTGGTATCAGGACTTGAGAGATGGAG(+AAAAAA)GATCCAAAAGTTTAGGGTCAGTCTGGGTATA NGG 10 8 17 8 24 0 100 5 10
    240606152911_t8kr TGCTGTATACCCAGACTGACCCTAAACTTTTG(+AAAAAA)GATCCTCCATCTCTCAAGTCCTGATACCACA NGG 10 8 17 8 24 0 100 5 10
    240606152909_ptq7 TCCTGCCAGCCCTTGAGCCTCTGCTCTCCTGG(+AAAAAA)GTACCCTCCTGGAACACCATGCTAACCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606152908_taob CTCGGGGAAGGTTAGCATGGTGTTCCAGGAGG(+AAAAAA)GTACCCAGGAGAGCAGAGGCTCAAGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606152906_w0fh GTGTCCTGTTTGATGTCTGAGGTCTGCTTTGG(+AAAAAA)GTACCGCCCTGGGAACTGCTAACCTCCGATT NGG 10 8 17 8 24 0 100 5 10
    240606152904_bdhy GGACCAATCGGAGGTTAGCAGTTCCCAGGGCG(+AAAAAA)GTACCCAAAGCAGACCTCAGACATCAAACAG NGG 10 8 17 8 24 0 100 5 10