Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152932_4el1 GGATAAAGGACACTTGGGAACGTGTGGCAGTG(+AAAAAA)GTACCTGAGCACAGGTAATTAGCCATGTGGC NGG 10 8 17 8 24 0 100 5 10
    240606152931_ufhs ATTTCGCCACATGGCTAATTACCTGTGCTCAG(+AAAAAA)GTACCACTGCCACACGTTCCCAAGTGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606152929_f4n4 CTCCTCTTTGGGGGTGCTGCATTCAAGCCTGG(+AAAAAA)GTACCTCCAAGAAGAGATCACAGCCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606152927_ntq1 AACTACCAACAGGCTGTGATCTCTTCTTGGAG(+AAAAAA)GTACCCAGGCTTGAATGCAGCACCCCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606152926_r5cs AATGTGCCCATGCCGACCGTGTTAGTCTGTAG(+AAAAAA)GTACCGCACTCACCCCATACCTTGGGCGTCT NGG 10 8 17 8 24 0 100 5 10
    240606152925_ap1z GATGCAGACGCCCAAGGTATGGGGTGAGTGCG(+AAAAAA)GTACCTACAGACTAACACGGTCGGCATGGGC NGG 10 8 17 8 24 0 100 5 10
    240606152924_yw1f GGGAGGGGCACGAAGTGGGGAACGGGACACAG(+AAAAAA)GATCCTAAACTCCAAGGGGACTGTCCACCTA NGG 10 8 17 8 24 0 100 5 10
    240606152922_hsnz GTCTGTAGGTGGACAGTCCCCTTGGAGTTTAG(+AAAAAA)GATCCTGTGTCCCGTTCCCCACTTCGTGCCC NGG 10 8 17 8 24 0 100 5 10
    240606152921_jf2w GGTGCTGTTGGCACCACCACCCGAAGACATGG(+AAAAAA)GTACCTGTACCAGACTTCATAAGCAGCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606152920_gibd TGGAGATGAGCTGCTTATGAAGTCTGGTACAG(+AAAAAA)GTACCCATGTCTTCGGGTGGTGGTGCCAACA NGG 10 8 17 8 24 0 100 5 10