Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153005_f2ea AAAATGAATTGATGTCATGTTTAGAAGAATAG(+AAAAAA)GTACCATGGATGAAAATGAAATGCACATAAG NGG 10 8 17 8 24 0 100 5 10
    240606153003_xgwl AAACACTTATGTGCATTTCATTTTCATCCATG(+AAAAAA)GTACCTATTCTTCTAAACATGACATCAATTC NGG 10 8 17 8 24 0 100 5 10
    240606153001_p1wz GCAAACAGCAAGAGGCTTTATTGAGAACACGG(+AAAAAA)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240606152958_m1xr GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+AAAAAA)GTACCCGTGTTCTCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606152956_ig6i CACACGTGGCCTTTTACCGTGATGCTCCTGTG(+AAAAAA)GTACCTGCAGCTTCCGAGGGGAACTTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606152955_8ck8 GACCCCACAGAAGTTCCCCTCGGAAGCTGCAG(+AAAAAA)GTACCACAGGAGCATCACGGTAAAAGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606152953_ttv2 GGGGAATTGCTAGGAGCAACTATGGCTGCCAG(+AAAAAA)GTACCCACCCCTCATCCTGGGCTCCCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240606152951_whvd TCCTGGGCCAGGGAGCCCAGGATGAGGGGTGG(+AAAAAA)GTACCTGGCAGCCATAGTTGCTCCTAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240606152950_kav7 GGGCCACGGCATTACAAGAGGGGCTGAAGTGG(+AAAAAA)GTACCAGGCTTTTCCTCATTTTTGGTACAAG NGG 10 8 17 8 24 0 100 5 10
    240606152948_wf3e GATCACTTGTACCAAAAATGAGGAAAAGCCTG(+AAAAAA)GTACCCACTTCAGCCCCTCTTGTAATGCCGT NGG 10 8 17 8 24 0 100 5 10