Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152534_r9c1 AAAATTTGAAAATATTGACTCCTGAGTGGGAG(+AAAAAA)GTACCACACCTGAACCAACCCACAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606152533_wyff CCGGCCAGCGTTTGTGGGTTGGTTCAGGTGTG(+AAAAAA)GTACCTCCCACTCAGGAGTCAATATTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606152531_t7az AATGTATCAAATCATGAAGCAAGGTGGCCAAG(+AAAAAA)GATCCCATAGCCTGGTCACAGTGGGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606152529_ll1k GAGGTCTGCTTCCCACTGTGACCAGGCTATGG(+AAAAAA)GATCCTTGGCCACCTTGCTTCATGATTTGAT NGG 10 8 17 8 24 0 100 5 10
    240606152528_qi3m ATTTTAGATGTTTAAGATAAGAAACTAGCTTG(+AAAAAA)GTACCATTAAAATGCCCACTATGTCCTACTG NGG 10 8 17 8 24 0 100 5 10
    240606152527_cyfe GTGATCAGTAGGACATAGTGGGCATTTTAATG(+AAAAAA)GTACCAAGCTAGTTTCTTATCTTAAACATCT NGG 10 8 17 8 24 0 100 5 10
    240606152525_vcai CAGTTTTCCGCATGATGTCTTCTGTGATGCAG(+AAAAAA)GTACCCTTATTCATTTGTGATTTTCAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606152524_7gsh GTGCTATTCTTGAAAATCACAAATGAATAAGG(+AAAAAA)GTACCTGCATCACAGAAGACATCATGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240606152522_j4l6 TTTGCTATAGTCACTAGGGCCATTTACTATAG(+AAAAAA)GATCCATTGTAATGAGAATGTTCATAAACTG NGG 10 8 17 8 24 0 100 5 10
    240606152521_iub8 CATGTCAGTTTATGAACATTCTCATTACAATG(+AAAAAA)GATCCTATAGTAAATGGCCCTAGTGACTATA NGG 10 8 17 8 24 0 100 5 10