Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152449_0wce AGAGCCAAAGAGCCAGGAGCTAAGCCCAAAGG(+AAAAAA)GATCCACAAACTGCAGCCGACCCGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606152448_o8qe TTAAATCCACTGCGGGTCGGCTGCAGTTTGTG(+AAAAAA)GATCCCTTTGGGCTTAGCTCCTGGCTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606152447_z1fp TCCAACCACGGCACCGGAAAAGGCTGTGCAAG(+AAAAAA)GATCCAACGCAGAGGCTGCTAGAGCGGATGC NGG 10 8 17 8 24 0 100 5 10
    240606152446_8ggf TCGAAGCATCCGCTCTAGCAGCCTCTGCGTTG(+AAAAAA)GATCCTTGCACAGCCTTTTCCGGTGCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240606152445_5dtm AGCCCTCTTCTGGCCTTCTCAGGGCCTTTTTG(+AAAAAA)GATCCCTGAAACAACACATTCCCTGACCTTT NGG 10 8 17 8 24 0 100 5 10
    240606152443_nl89 GACAGAAAGGTCAGGGAATGTGTTGTTTCAGG(+AAAAAA)GATCCAAAAAGGCCCTGAGAAGGCCAGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606152441_pl0y TGCATACCTTCCTTCAAGTTTTGTCCTTGTAG(+AAAAAA)GTACCCTCCTTTTGATTAAGAGGTGGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606152439_w0pf TACACAACCTCCACCTCTTAATCAAAAGGAGG(+AAAAAA)GTACCTACAAGGACAAAACTTGAAGGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606152438_ov9d CTACCCATATCCAGCAGCTCTGCCTTTCTCAG(+AAAAAA)GTACCTCCTGAAGGTCCTGCCATCCTGCCGA NGG 10 8 17 8 24 0 100 5 10
    240606152437_0cyu AAGACTCGGCAGGATGGCAGGACCTTCAGGAG(+AAAAAA)GTACCTGAGAAAGGCAGAGCTGCTGGATATG NGG 10 8 17 8 24 0 100 5 10