Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152350_yi1i TGCAGGGTAAGATACCCATATACATTTTCCTG(+AAAAAA)GTACCATTTCCTCACTCAGAACAGTGACAGA NGG 10 8 17 8 24 0 100 5 10
    240606152349_6qj3 TCAAATCTGTCACTGTTCTGAGTGAGGAAATG(+AAAAAA)GTACCAGGAAAATGTATATGGGTATCTTACC NGG 10 8 17 8 24 0 100 5 10
    240606152348_gda1 CCTGTTCATTAGTTTCTGACTCCTTGGGTAAG(+AAAAAA)GATCCATTAACATAAGCTCTAAAGTGAAATT NGG 10 8 17 8 24 0 100 5 10
    240606152347_rybx TTTTTAATTTCACTTTAGAGCTTATGTTAATG(+AAAAAA)GATCCTTACCCAAGGAGTCAGAAACTAATGA NGG 10 8 17 8 24 0 100 5 10
    240606152346_ram1 AACAGCTCCTCTGAAAGGTTAATTAAGAGGTG(+AAAAAA)GTACCCAAATCAAGCCAAAATTTTATTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606152344_z99z TACATTTGAAATAAAATTTTGGCTTGATTTGG(+AAAAAA)GTACCACCTCTTAATTAACCTTTCAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606152342_2z5w TCCTCGGGGCGGAGGCTGCCCTTCTCAGGCAG(+AAAAAA)GATCCTTCTGATCCATGGAACTTCCTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606152341_i8l1 TGTGGCACAAAGGAAGTTCCATGGATCAGAAG(+AAAAAA)GATCCTGCCTGAGAAGGGCAGCCTCCGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606152340_w277 AGCATTTCTGAAGGTGGGAGATTCCTAATCTG(+AAAAAA)GATCCAGTTGAACTACAAGTACTGGATTCGG NGG 10 8 17 8 24 0 100 5 10
    240606152339_9dox TCATGCCGAATCCAGTACTTGTAGTTCAACTG(+AAAAAA)GATCCAGATTAGGAATCTCCCACCTTCAGAA NGG 10 8 17 8 24 0 100 5 10