Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152324_950o CAGGGAGGCTTGGTAGTTTCTCTGTGTAATAG(+AAAAAA)GATCCAGTGAACTTCAGGTTTCTCCTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606152323_pnl1 GGAGAAACAAAGGAGAAACCTGAAGTTCACTG(+AAAAAA)GATCCTATTACACAGAGAAACTACCAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606152319_n3tb GAGACTCCTGCAAAGGCTATGAAACTCCCAGG(+AAAAAA)GATCCCAGAGGGCTGGAATCCTGAGATGTCC NGG 10 8 17 8 24 0 100 5 10
    240606152317_6rlx ATGCCGGACATCTCAGGATTCCAGCCCTCTGG(+AAAAAA)GATCCCTGGGAGTTTCATAGCCTTTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606152316_mdwc GGTCTTATATGATAATAACTACAGTAAGTGTG(+AAAAAA)GTACCCAGACTGAAAGATCAATACATGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606152315_j121 GACATAGAGCATGTATTGATCTTTCAGTCTGG(+AAAAAA)GTACCACACTTACTGTAGTTATTATCATATA NGG 10 8 17 8 24 0 100 5 10
    240606152312_owks TATTAGAGCAAGAGGCAGAGACTATGACCCAG(+AAAAAA)GTACCCCAGGGAAGATTCAGTCTAGAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240606152310_ozrq CAAAATTACCTCTAGACTGAATCTTCCCTGGG(+AAAAAA)GTACCTGGGTCATAGTCTCTGCCTCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606152308_76dx GGCCCCAGAGGGAGGTGCAATCGACCTTTAGG(+AAAAAA)GATCCTCACCGACCAACTCAGCGAGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240606152307_cfim TCCTCTTCTGTCTCGCTGAGTTGGTCGGTGAG(+AAAAAA)GATCCCTAAAGGTCGATTGCACCTCCCTCTG NGG 10 8 17 8 24 0 100 5 10