Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153500_4d3m ACGCGCTTTGGAAGATTCTCCTTTTGCCTGTG(+AAAAAA)GATCCTTTCTCCCCTAAAGAAAGCTATTAAC NGG 10 8 17 8 24 0 100 5 10
    240606153458_2rzu GAGTGCTGTGCAGGTGTATGTGCAATGCATGG(+AAAAAA)GTACCCTATGAAACTTGTTGGGGTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606153456_s4s4 GAATCTCTAGAAACCCCAACAAGTTTCATAGG(+AAAAAA)GTACCCATGCATTGCACATACACCTGCACAG NGG 10 8 17 8 24 0 100 5 10
    240606153454_htuc CAGCCCAAGCAGCTCTGAGAAAGGGTTCCCTG(+AAAAAA)GATCCTTCCTGAGCTGTGTTCTTTACTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606153453_gab8 AGTCCCCTGAGTAAAGAACACAGCTCAGGAAG(+AAAAAA)GATCCAGGGAACCCTTTCTCAGAGCTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606153451_rd6v TGGTGGAAACATCCTTCCAACAAGTGTAGTGG(+AAAAAA)GATCCCGGGGCCCAGGGCAGGCCGAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606153448_xltg GCAGGCCAGCCTCGGCCTGCCCTGGGCCCCGG(+AAAAAA)GATCCCACTACACTTGTTGGAAGGATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240606153446_pvgo ATGGACTCCGAGGGCGTGGGGTGGGCTCCAAG(+AAAAAA)GATCCTGGGCATCCGGATGGCATCGGCTACC NGG 10 8 17 8 24 0 100 5 10
    240606153444_8aw8 TATGTGGTAGCCGATGCCATCCGGATGCCCAG(+AAAAAA)GATCCTTGGAGCCCACCCCACGCCCTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240606153443_32jh TTAATTCCAAGCCACACATGATGGTGGCACAG(+AAAAAA)GATCCTAACCCCAGTGCTTGGAAGGCTACGG NGG 10 8 17 8 24 0 100 5 10