Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152217_g26k TTGTTTGGGTTTGTTCTAGGTATGGTCCCAGG(+AAAAAA)GATCCCAGATCAAACCAGGCCCCTGGGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606152215_5o8h TAGGATAGGCCCAGGGGCCTGGTTTGATCTGG(+AAAAAA)GATCCCTGGGACCATACCTAGAACAAACCCA NGG 10 8 17 8 24 0 100 5 10
    240606152213_vj7e ATGGGAAGATAATCTTAGAGCTACTGTTTATG(+AAAAAA)GATCCAGTGTGAGTTGTTGGTGTGGAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240606152211_2s85 CTGAGACTCCTCCACACCAACAACTCACACTG(+AAAAAA)GATCCATAAACAGTAGCTCTAAGATTATCTT NGG 10 8 17 8 24 0 100 5 10
    240606152208_waiz TCTGCAAATGTTATGCCCCAGTGAGACCAAGG(+AAAAAA)GTACCATTTCCTAAGAAGCAGGCAGAGTCTT NGG 10 8 17 8 24 0 100 5 10
    240606152207_e3wa GAGCAAAGACTCTGCCTGCTTCTTAGGAAATG(+AAAAAA)GTACCCTTGGTCTCACTGGGGCATAACATTT NGG 10 8 17 8 24 0 100 5 10
    240606152206_5led GGCCCCCAATACAGGACTGACTCTGAGTTCTG(+AAAAAA)GTACCCACTTCCAAATGACTCTGGAACCTAA NGG 10 8 17 8 24 0 100 5 10
    240606152204_qp7v GGGCCTTAGGTTCCAGAGTCATTTGGAAGTGG(+AAAAAA)GTACCAGAACTCAGAGTCAGTCCTGTATTGG NGG 10 8 17 8 24 0 100 5 10
    240606152203_mqqf ATTTTTGAAACTTTGCATTGTACAGATACATG(+AAAAAA)GTACCTTTGTTCTGGTTACCTAATTTTATAT NGG 10 8 17 8 24 0 100 5 10
    240606152202_75sh TATATATATAAAATTAGGTAACCAGAACAAAG(+AAAAAA)GTACCATGTATCTGTACAATGCAAAGTTTCA NGG 10 8 17 8 24 0 100 5 10