Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152111_x11q AATTTTTCTTTATCTCCTCTTTAAATGCCCTG(+AAAAAA)GTACCTCAGCATCAGAGCCTCTCCCAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606152109_8i2e AGCTGTCAGCTGGGAGAGGCTCTGATGCTGAG(+AAAAAA)GTACCAGGGCATTTAAAGAGGAGATAAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606152108_4h1c CACAGGATAAATCCATGGTAATTCCTATAAAG(+AAAAAA)GTACCTACTGCTCCCATGAGCCAGCCCGAAA NGG 10 8 17 8 24 0 100 5 10
    240606152107_h5cc ACAGATTTCGGGCTGGCTCATGGGAGCAGTAG(+AAAAAA)GTACCTTTATAGGAATTACCATGGATTTATC NGG 10 8 17 8 24 0 100 5 10
    240606152105_kwaq AAATCCCAGCACCGGGAAAGAGGAGCCAGGAG(+AAAAAA)GATCCTCTGGAGCCTGTTGGCAAGTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606152103_q9p4 TAGCTAGAAAGACTTGCCAACAGGCTCCAGAG(+AAAAAA)GATCCTCCTGGCTCCTCTTTCCCGGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606152101_fv11 CCCTTGAGGGGCGCACCCCCCAAAGGCTCCCG(+AAAAAA)GATCCCCTAAAGTTTCACAGCACCTCTGACT NGG 10 8 17 8 24 0 100 5 10
    240606152059_9p5q GTACAAGTCAGAGGTGCTGTGAAACTTTAGGG(+AAAAAA)GATCCGGGAGCCTTTGGGGGGTGCGCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606152057_pjo3 TTTAAAAGTGCAAAGTCTCTCTGCAAATGGTG(+AAAAAA)GATCCTGTATGGGATAGTCCCCTTCTCATAG NGG 10 8 17 8 24 0 100 5 10
    240606152056_ml0s AACTCCTATGAGAAGGGGACTATCCCATACAG(+AAAAAA)GATCCACCATTTGCAGAGAGACTTTGCACTT NGG 10 8 17 8 24 0 100 5 10