Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152032_49c5 ATGACACTGTCCAGAGATGTCCTCCCAGACTG(+AAAAAA)GTACCTTCGTAAACACACAAGCAGGGTACAA NGG 10 8 17 8 24 0 100 5 10
    240606152031_pxpw GGCTTTTGTACCCTGCTTGTGTGTTTACGAAG(+AAAAAA)GTACCAGTCTGGGAGGACATCTCTGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240606152029_utlz ACAATTTGGGCAGTGCCATTCCTTAGGCTTGG(+AAAAAA)GTACCAGGCTGCACAATAGCACAGAGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606152027_ipoq TCATCTAAAGCTCTGTGCTATTGTGCAGCCTG(+AAAAAA)GTACCCAAGCCTAAGGAATGGCACTGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240606152026_w8sb TTGACATTAGGGAACTAAGTTGAGAGATTGAG(+AAAAAA)GATCCCCAAATCAACAACCAAGAAAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240606152024_u9xc TCCTTCAGAATTTTCTTGGTTGTTGATTTGGG(+AAAAAA)GATCCTCAATCTCTCAACTTAGTTCCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240606152022_6cel TAGAAGTGACCTGGAGGGAGTCTTCACAGAGG(+AAAAAA)GATCCTCTAGATGAAGTTCTCCTGTGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606152021_2njw TAGACAGGCTCACAGGAGAACTTCATCTAGAG(+AAAAAA)GATCCCTCTGTGAAGACTCCCTCCAGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240606152019_q1lx AGCAACAATGAACCACTGCCCTCATTGTCAGG(+AAAAAA)GATCCACGACTTTCCTGGCCTTGCAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606152018_lje7 AAGCTCATGCTTGCAAGGCCAGGAAAGTCGTG(+AAAAAA)GATCCCTGACAATGAGGGCAGTGGTTCATTG NGG 10 8 17 8 24 0 100 5 10