Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151945_3atj TTTAGCCTTTGTGGGAAAGAGTAAGGCATGTG(+AAAAAA)GATCCGACCAGGTGGTAAGGTTGTAAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606151944_u3q2 GACAAGTTCCTTACAACCTTACCACCTGGTCG(+AAAAAA)GATCCACATGCCTTACTCTTTCCCACAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606151942_ooie CTATAAAATGAGGTATGTGAGGAGGACTGTGG(+AAAAAA)GTACCTGGGCACAAGATTTCTGAAACCGGTA NGG 10 8 17 8 24 0 100 5 10
    240606151939_i633 GTGGATACCGGTTTCAGAAATCTTGTGCCCAG(+AAAAAA)GTACCCACAGTCCTCCTCACATACCTCATTT NGG 10 8 17 8 24 0 100 5 10
    240606151938_804g ATGCCACTGTCCAGAGATGTTCTTCCAGAATG(+AAAAAA)GTACCTTCATAAACACACAAGCAGGATCCAA NGG 10 8 17 8 24 0 100 5 10
    240606151937_dnj9 GGCTTTTGGATCCTGCTTGTGTGTTTATGAAG(+AAAAAA)GTACCATTCTGGAAGAACATCTCTGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240606151935_96pc ACAATTTGAGCAGTGCCATTCCTTAGGCTTGG(+AAAAAA)GTACCAGGCTGCACAATAGCACAGAGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606151933_85x5 TCATCTAAAGCTCTGTGCTATTGTGCAGCCTG(+AAAAAA)GTACCCAAGCCTAAGGAATGGCACTGCTCAA NGG 10 8 17 8 24 0 100 5 10
    240606151932_dzzf CCAGAATGGTACCTTCATAAACACACAAGCAG(+AAAAAA)GATCCAAAAGCCCCAAAAGCCCTCTGAAATT NGG 10 8 17 8 24 0 100 5 10
    240606151931_0stg AAGAAAATTTCAGAGGGCTTTTGGGGCTTTTG(+AAAAAA)GATCCTGCTTGTGTGTTTATGAAGGTACCAT NGG 10 8 17 8 24 0 100 5 10