Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151840_3v88 CTTTGTGGTAAGTCTTTGCTTCAGACTGTTGG(+AAAAAA)GTACCCAGAACGAGAGCTATAAAAATGCATT NGG 10 8 17 8 24 0 100 5 10
    240606151838_7uil TGAACAATGCATTTTTATAGCTCTCGTTCTGG(+AAAAAA)GTACCCAACAGTCTGAAGCAAAGACTTACCA NGG 10 8 17 8 24 0 100 5 10
    240606151836_j2rl CTTTATTTGGATAGCAGCGCTTCAGTTCCTAG(+AAAAAA)GATCCTTTTATAAATGGTAAAACCCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240606151835_i7qv TTGTAAATCCAGGGTTTTACCATTTATAAAAG(+AAAAAA)GATCCTAGGAACTGAAGCGCTGCTATCCAAA NGG 10 8 17 8 24 0 100 5 10
    240606151834_u8mu CTGACAAAGAGGTGGGCCAGGATGGGACATGG(+AAAAAA)GTACCAGGAGTGCACAGGCTTGGGCAATTTG NGG 10 8 17 8 24 0 100 5 10
    240606151832_54k3 ATACACAAATTGCCCAAGCCTGTGCACTCCTG(+AAAAAA)GTACCCATGTCCCATCCTGGCCCACCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606151831_p3q7 TATTAAAGGCGAGGGGAGAGAAATTGGGGAAG(+AAAAAA)GTACCTGCGTTTTATAAATAGCCTCAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606151829_9rzc AGTGGCTGCCTGAGGCTATTTATAAAACGCAG(+AAAAAA)GTACCTTCCCCAATTTCTCTCCCCTCGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606151828_vkes GCAGGACATGGAGGGTGGGCATGTGCCTGTGG(+AAAAAA)GTACCAACACTCCACCTTTGACCTTCACACT NGG 10 8 17 8 24 0 100 5 10
    240606151827_h32i GTTCAAGTGTGAAGGTCAAAGGTGGAGTGTTG(+AAAAAA)GTACCCACAGGCACATGCCCACCCTCCATGT NGG 10 8 17 8 24 0 100 5 10