Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151930_yu92 TTGACATTAGAGAACTAAGTTGAGAGATTGAG(+AAAAAA)GATCCCAAATCAACAACCAAGAAAATTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606151928_pvsr GTCCTTCAGAATTTTCTTGGTTGTTGATTTGG(+AAAAAA)GATCCTCAATCTCTCAACTTAGTTCTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240606151926_ragw GAAGAGTCTCTGTGTGGGACATGGCAGCCAAG(+AAAAAA)GATCCCTCAGCCCTGTCAGCACTTCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606151924_xvp9 AATAGAGCCCTGAAGTGCTGACAGGGCTGAGG(+AAAAAA)GATCCTTGGCTGCCATGTCCCACACAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240606151923_dhuo AGCAACAATGAACCACTGCCCTCATTGTCAGG(+AAAAAA)GATCCACGACTTTCCTGGCCTTGCAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606151922_2nh3 AAGCTCATGCTTGCAAGGCCAGGAAAGTCGTG(+AAAAAA)GATCCCTGACAATGAGGGCAGTGGTTCATTG NGG 10 8 17 8 24 0 100 5 10
    240606151920_vfot TCATCTAAAGCTCTGTGCTATTGTGCAGCCTG(+AAAAAA)GTACCCAAGCCTAAGGAATGGCACTGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240606151918_xfo9 ACAATTTGGGCAGTGCCATTCCTTAGGCTTGG(+AAAAAA)GTACCAGGCTGCACAATAGCACAGAGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606151916_qnx1 GGCTTTTCGACCCTGCTTGTGTGTTTACGAAG(+AAAAAA)GTACCGGTCTGGGAGGACATCTCTGGACAGC NGG 10 8 17 8 24 0 100 5 10
    240606151914_ssfo ATGCCGCTGTCCAGAGATGTCCTCCCAGACCG(+AAAAAA)GTACCTTCGTAAACACACAAGCAGGGTCGAA NGG 10 8 17 8 24 0 100 5 10