Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153234_8qby TCAGACATACACACAGAGGCCAGAGAATGCTG(+AAAAAA)GATCCCCTGAGTTGTGCTCGTTGTGAGCTAC NGG 10 8 17 8 24 0 100 5 10
    240606153233_972w ATCATACCTGGAATGCATGCAGCCTGCTGTTG(+AAAAAA)GTACCCTGCTCACCTGTGTCTTTCTTACCCA NGG 10 8 17 8 24 0 100 5 10
    240606153231_9uta CCTCCTGGGTAAGAAAGACACAGGTGAGCAGG(+AAAAAA)GTACCAACAGCAGGCTGCATGCATTCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606153229_msw2 TAAACCCAGTCCTTAGGAGACTGAGGCAGGAG(+AAAAAA)GATCCCTGTGAGTTCCAGGCCAGTCTAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606153228_eiyy TCTGCAGCTTAGACTGGCCTGGAACTCACAGG(+AAAAAA)GATCCTCCTGCCTCAGTCTCCTAAGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240606153226_zhb4 TACCCAGCCTTTCAAAATTCTTTTAAATTTAG(+AAAAAA)GTACCCTTCTAAAGAAGGCCCTGGTTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240606153225_2bjs ATTGATAAGAAACCAGGGCCTTCTTTAGAAGG(+AAAAAA)GTACCTAAATTTAAAAGAATTTTGAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606153223_pmiq TCCTCACTGTCCAAGTCTGATATGCTCCATCG(+AAAAAA)GATCCGCAGTTCAGGGGAAAGGTCACAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606153222_jzhh ACATGGCTCTGTGACCTTTCCCCTGAACTGCG(+AAAAAA)GATCCGATGGAGCATATCAGACTTGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240606153219_zxzg ATCAACTGCATTAAGTTGCCAGCACCTGCCGG(+AAAAAA)GATCCACGAGACAGAGAAACACTAAGCAATA NGG 10 8 17 8 24 0 100 5 10