Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151959_y7kh TCCTTCAGAATTTTCTTGGTTGTTGATTTGGG(+AAAAAA)GATCCTCAATCTCTCAACTTAGTTCCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240606151958_pt8g TTGACATTAGGGAACTAAGTTGAGAGATTGAG(+AAAAAA)GATCCCCAAATCAACAACCAAGAAAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240606151957_ma8j TTGCTAGAGGAAAAATAGACAGAGAATCTAAG(+AAAAAA)GTACCCCTGTCCCCAGAGCATGGTCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240606151955_br69 CCCGAGGAAATGACCATGCTCTGGGGACAGGG(+AAAAAA)GTACCTTAGATTCTCTGTCTATTTTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606151953_9sx5 ACATCCACCATATGCCTGCTCCCCTGTCGCCG(+AAAAAA)GTACCAAGTGAGATGTCTGTGTGGCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240606151952_jglc TGCAAAAACAAGCCACACAGACATCTCACTTG(+AAAAAA)GTACCGGCGACAGGGGAGCAGGCATATGGTG NGG 10 8 17 8 24 0 100 5 10
    240606151950_nr01 TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+AAAAAA)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606151949_gokp TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+AAAAAA)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606151948_q2a8 TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+AAAAAA)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606151946_rvd9 TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+AAAAAA)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10