Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153308_71ma CTATTTCTTTCCATATTTTAGGGTAGGCTGTG(+AAAAAA)GATCCAAGGAAACCTGTTGTTTTATGCATGA NGG 10 8 17 8 24 0 100 5 10
    240606153306_q0be CATCCTTGTCACTGGCACCCCTGCTGTGATGG(+AAAAAA)GTACCTGGCAGCTTCCAAAATCCAAACTCTC NGG 10 8 17 8 24 0 100 5 10
    240606153304_rrej TTGCAGAGAGTTTGGATTTTGGAAGCTGCCAG(+AAAAAA)GTACCCATCACAGCAGGGGTGCCAGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240606153303_xmxe CCTAAACAACAAAATTCATACATGAAAAAACG(+AAAAAA)GTACCACAGGTTTTGGTGTCCTCACTAACAG NGG 10 8 17 8 24 0 100 5 10
    240606153301_mcee GACATCTGTTAGTGAGGACACCAAAACCTGTG(+AAAAAA)GTACCGTTTTTTCATGTATGAATTTTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606153258_lakm CTGAAGATGATATTTTGATTTGTATTTTGGGG(+AAAAAA)GTACCTGTGTTGAGTCGATAAAACATTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606153257_yajc GGAGATGAAAATGTTTTATCGACTCAACACAG(+AAAAAA)GTACCCCCAAAATACAAATCAAAATATCATC NGG 10 8 17 8 24 0 100 5 10
    240606153255_7ugi AACCTCTCCCTAGCAGGCTTCCCTGGGACGGG(+AAAAAA)GTACCTTTCCAGTTGGTGTTCCCTGGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606153254_rstx CACACACAAGCCAGGGAACACCAACTGGAAAG(+AAAAAA)GTACCCCGTCCCAGGGAAGCCTGCTAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606153252_9to4 CGTGTGTTCCTGCCAGCATAGGACCAGGATGG(+AAAAAA)GTACCTTCTCCAAGCTCCGCAGAGGAGGACC NGG 10 8 17 8 24 0 100 5 10