Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152249_d2ow TATTACATATTTCTTACGGAATAGTGGGTTAG(+AAAAAA)GTACCCAATTTCTCATCTCACTTCGTAATTT NGG 10 8 17 8 24 0 100 5 10
    240606152247_38sz CATACAAATTACGAAGTGAGATGAGAAATTGG(+AAAAAA)GTACCTAACCCACTATTCCGTAAGAAATATG NGG 10 8 17 8 24 0 100 5 10
    240606152246_ye0d ACATTTGTTAACAAAATGCATTTATTTGGAAG(+AAAAAA)GATCCAGTTATCAGTTGTCAAAAGCTCTTAT NGG 10 8 17 8 24 0 100 5 10
    240606152244_xoum AACATATAAGAGCTTTTGACAACTGATAACTG(+AAAAAA)GATCCTTCCAAATAAATGCATTTTGTTAACA NGG 10 8 17 8 24 0 100 5 10
    240606152243_lxiy TGTCTTATTTGCCAGCTGAGAAAATGTACATG(+AAAAAA)GTACCATCATTAAGGTGGTCGGTATATAGGA NGG 10 8 17 8 24 0 100 5 10
    240606152242_czw5 TTTTTTCCTATATACCGACCACCTTAATGATG(+AAAAAA)GTACCATGTACATTTTCTCAGCTGGCAAATA NGG 10 8 17 8 24 0 100 5 10
    240606152241_v6r3 CTCTGCTTTCTCGGGGTTGCCACAGCAGACTG(+AAAAAA)GTACCTCATTTCACCACACCACTTAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240606152240_m7kx ACACCTGTTCTTAAGTGGTGTGGTGAAATGAG(+AAAAAA)GTACCAGTCTGCTGTGGCAACCCCGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606152236_ei66 ATTGCTTTAACCCAAGTTAAAACCAAGCTTGG(+AAAAAA)GATCCCATGCTTGCTGAGATGATTGACGGCT NGG 10 8 17 8 24 0 100 5 10
    240606152235_dxi9 ATGACAGCCGTCAATCATCTCAGCAAGCATGG(+AAAAAA)GATCCCAAGCTTGGTTTTAACTTGGGTTAAA NGG 10 8 17 8 24 0 100 5 10