Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152339_9dox TCATGCCGAATCCAGTACTTGTAGTTCAACTG(+AAAAAA)GATCCAGATTAGGAATCTCCCACCTTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240606152338_ay4r CCCCTAATTATTATTATTTTTTACATGTGATG(+AAAAAA)GTACCATAACATTTCTGGGACTTCTACAGCA NGG 10 8 17 8 24 0 100 5 10
    240606152336_vtv9 TACATTGCTGTAGAAGTCCCAGAAATGTTATG(+AAAAAA)GTACCATCACATGTAAAAAATAATAATAATT NGG 10 8 17 8 24 0 100 5 10
    240606152335_v2xg TAGTTTATAAACAGGCATCCTGGAGTTAGCAG(+AAAAAA)GTACCTCTCCCATAGCATTATATTTTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240606152334_198n ATAATTTTGGAAAATATAATGCTATGGGAGAG(+AAAAAA)GTACCTGCTAACTCCAGGATGCCTGTTTATA NGG 10 8 17 8 24 0 100 5 10
    240606152332_1o12 CAGCCTGATACAACAGGCAGTGTCCTTCTTCG(+AAAAAA)GATCCCATCTGGGGATGCTGCTCGCCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606152331_paxw GAGGTGTCTGGGCGAGCAGCATCCCCAGATGG(+AAAAAA)GATCCGAAGAAGGACACTGCCTGTTGTATCA NGG 10 8 17 8 24 0 100 5 10
    240606152329_ywer TATCACATAGTCGCAGGTCATAAAGGAATGGG(+AAAAAA)GATCCAAAAGCAGTTTTCATCTTCAGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606152328_icma CTCTAAGACTCTGAAGATGAAAACTGCTTTTG(+AAAAAA)GATCCCCATTCCTTTATGACCTGCGACTATG NGG 10 8 17 8 24 0 100 5 10
    240606152326_mz63 TCTCTCTTGTAACTTAGAATACAATGAAAATG(+AAAAAA)GATCCAGCCATCATGGGGTTATTGGGAAAGT NGG 10 8 17 8 24 0 100 5 10