Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152405_r866 AGCTCAGTGATAGCACTTGCCTCGGGTGCACG(+AAAAAA)GTACCCTGGTTTAGATGCCCTGCACTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606152403_an8i ATTTTCTTTCAGTGCAGGGCATCTAAACCAGG(+AAAAAA)GTACCGTGCACCCGAGGCAAGTGCTATCACT NGG 10 8 17 8 24 0 100 5 10
    240606152401_2wcu CTTAATCAGCTCACCCGCTCACAGGGTATCTG(+AAAAAA)GTACCTTAACTCCTGCCGGGTCTGAGTATTG NGG 10 8 17 8 24 0 100 5 10
    240606152400_6gdq ATTTACAATACTCAGACCCGGCAGGAGTTAAG(+AAAAAA)GTACCAGATACCCTGTGAGCGGGTGAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606152359_vzzd ACCTCATACAAAACTTGTTCAACAGTCTAGTG(+AAAAAA)GTACCAACAGCGTTGAGATGGCAGTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240606152358_hbzq TGTAGCGCAAAACTGCCATCTCAACGCTGTTG(+AAAAAA)GTACCACTAGACTGTTGAACAAGTTTTGTAT NGG 10 8 17 8 24 0 100 5 10
    240606152356_1dg0 TGATCATTATGACAGGTCGCTGCCCTCCAGTG(+AAAAAA)GTACCACTGACCTACAGAAGGACATCATGGC NGG 10 8 17 8 24 0 100 5 10
    240606152355_ftiq GCTCTGCCATGATGTCCTTCTGTAGGTCAGTG(+AAAAAA)GTACCACTGGAGGGCAGCGACCTGTCATAAT NGG 10 8 17 8 24 0 100 5 10
    240606152353_tnom GCAGCTCAGGGTTGGGGCCTAGTTACAGTTTG(+AAAAAA)GATCCTTAGTCCAAGAATTTTGAGAACGTGA NGG 10 8 17 8 24 0 100 5 10
    240606152352_esde TTTACTCACGTTCTCAAAATTCTTGGACTAAG(+AAAAAA)GATCCAAACTGTAACTAGGCCCCAACCCTGA NGG 10 8 17 8 24 0 100 5 10