Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151658_yzel GGTAGGCTGTGCCTGCTAGACCTCCCCTGAAG(+AAAAAA)GTACCTTGCTGTTGTCCCTGATGCAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606151657_cd1q TGCCATCAGCCTGCATCAGGGACAACAGCAAG(+AAAAAA)GTACCTTCAGGGGAGGTCTAGCAGGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240606151656_u9ou GAGGGCAGGATTGGGCATGGGGATGCTGTAAG(+AAAAAA)GATCCAGCTTGTTTTGGCCACTTGGGATGGG NGG 10 8 17 8 24 0 100 5 10
    240606151654_svhn TGAGACCCATCCCAAGTGGCCAAAACAAGCTG(+AAAAAA)GATCCTTACAGCATCCCCATGCCCAATCCTG NGG 10 8 17 8 24 0 100 5 10
    240606151652_mz01 TTAGCTTACTCACCCTTTGTCCCCTCCCCCAG(+AAAAAA)GTACCATGTGCCGAGGCCTCAGACACAGCGT NGG 10 8 17 8 24 0 100 5 10
    240606151650_1j8h GGACTACGCTGTGTCTGAGGCCTCGGCACATG(+AAAAAA)GTACCTGGGGGAGGGGACAAAGGGTGAGTAA NGG 10 8 17 8 24 0 100 5 10
    240606151649_z47q CGGCGCTGTCCAGTCTAAACCCCTGGAGGTTG(+AAAAAA)GATCCTCAGCAGGGCTCCTGTTCTGGCGTCC NGG 10 8 17 8 24 0 100 5 10
    240606151648_optn TCATGGGACGCCAGAACAGGAGCCCTGCTGAG(+AAAAAA)GATCCAACCTCCAGGGGTTTAGACTGGACAG NGG 10 8 17 8 24 0 100 5 10
    240606151646_bpx1 CAGAGATGCCTGGCTGTCAGGACAGAAGGCCG(+AAAAAA)GTACCCCAAGGTCCTGCTCTCAGGCTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606151644_b918 CTTCTCTTCCAGCCTGAGAGCAGGACCTTGGG(+AAAAAA)GTACCGGCCTTCTGTCCTGACAGCCAGGCAT NGG 10 8 17 8 24 0 100 5 10