Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151643_tjxw CAGTTCTCCCTTAAGCCAGGCTAGATCACTGG(+AAAAAA)GTACCCAAGCCCCCTGCCACCCCTTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606151641_q6e0 CCTGAGCTGCCAAGGGGTGGCAGGGGGCTTGG(+AAAAAA)GTACCCAGTGATCTAGCCTGGCTTAAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606151640_3rj1 GTGGCTGGGAGAAAGATGGCCCTTGCATGCTG(+AAAAAA)GTACCACAGCCCAGACATCTCTGCTGCTCTA NGG 10 8 17 8 24 0 100 5 10
    240606151638_lk71 CAGAGTAGAGCAGCAGAGATGTCTGGGCTGTG(+AAAAAA)GTACCAGCATGCAAGGGCCATCTTTCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240606151637_330i AACCGAATTCTCGCTTGGCCTCTACCGTTCAG(+AAAAAA)GATCCCCTTCCCACCCACCCACCCCAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606151635_96i7 AAAGGTTTTTTGGGGTGGGTGGGTGGGAAGGG(+AAAAAA)GATCCTGAACGGTAGAGGCCAAGCGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240606151633_4yf6 TCTATCAGCTTTCCGGGGTGGGAGGGGCAGGG(+AAAAAA)GATCCAGCTGGCCTGGAACAGTGGCCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606151632_dqo3 CTGCAGACTTGGCCACTGTTCCAGGCCAGCTG(+AAAAAA)GATCCCCTGCCCCTCCCACCCCGGAAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606151631_khat GGGATAGGGGATGTACAGACCCAAGGGGACTG(+AAAAAA)GATCCTTTAAGCCACCACAAACTCGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240606151630_cest GACATCCAGTCCGAGTTTGTGGTGGCTTAAAG(+AAAAAA)GATCCAGTCCCCTTGGGTCTGTACATCCCCT NGG 10 8 17 8 24 0 100 5 10