Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151611_s3zy GCTGAGGGATGGCTCAGGGGTTAAGAGAACTG(+AAAAAA)GATCCTCTGCCACAGAGCCTTAGTTCCATTC NGG 10 8 17 8 24 0 100 5 10
    240606151610_8j2k ACTGGGAATGGAACTAAGGCTCTGTGGCAGAG(+AAAAAA)GATCCAGTTCTCTTAACCCCTGAGCCATCCC NGG 10 8 17 8 24 0 100 5 10
    240606151608_84m0 CCCTGCTTTGAGCTCTGCTACCTATGGGAGGG(+AAAAAA)GATCCACAGGTGCCCATCCTCCTCCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240606151606_u60z TGAAGTTCTAGGGAGGAGGATGGGCACCTGTG(+AAAAAA)GATCCCCTCCCATAGGTAGCAGAGCTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606151604_98am CTGCAGGTGCTGGTCCTGCAAAGTCCAAGCGG(+AAAAAA)GTACCCCTTCCTGGGTGATGACCCTGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606151602_kqg6 GCATTCTGCACAGGGTCATCACCCAGGAAGGG(+AAAAAA)GTACCCGCTTGGACTTTGCAGGACCAGCACC NGG 10 8 17 8 24 0 100 5 10
    240606151600_919a GTTGTGGGGTTCGTAGCAGTCCCAGGATCCTG(+AAAAAA)GTACCTAAGGCAGCTGGGACCCAGAACAGAG NGG 10 8 17 8 24 0 100 5 10
    240606151558_q67i GGAAACTCTGTTCTGGGTCCCAGCTGCCTTAG(+AAAAAA)GTACCAGGATCCTGGGACTGCTACGAACCCC NGG 10 8 17 8 24 0 100 5 10
    240606151555_cyut ACATAGAGTTGTGGGGTTCGTAGCAGTCCCAG(+AAAAAA)GATCCTGGTACCTAAGGCAGCTGGGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606151554_1nox CTGTTCTGGGTCCCAGCTGCCTTAGGTACCAG(+AAAAAA)GATCCTGGGACTGCTACGAACCCCACAACTC NGG 10 8 17 8 24 0 100 5 10