Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150224_yg90 AGCTTCTACCCATTCTCTCTCCCACTTCTGAG(+AAAAAA)GATCCCCACAGCTAACATGCAATGCCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606150223_ze84 GTAGGGAGCAGGCATTGCATGTTAGCTGTGGG(+AAAAAA)GATCCTCAGAAGTGGGAGAGAGAATGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240606150221_jf8h TTTACACTGTAGTATCTCTGTGATCACTGCTG(+AAAAAA)GATCCTCTGTATTCCGAAACAGGTACAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606150220_cvxy TGTCTGTTTTGTACCTGTTTCGGAATACAGAG(+AAAAAA)GATCCAGCAGTGATCACAGAGATACTACAGT NGG 10 8 17 8 24 0 100 5 10
    240606150218_a9j2 GCCTGAGATTCCATTTCTTCTTCTCTTCACAG(+AAAAAA)GTACCTGAATTCAGGTGTGTCTCTTGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606150217_1xxd AATCTCAGGGCAAGAGACACACCTGAATTCAG(+AAAAAA)GTACCTGTGAAGAGAAGAAGAAATGGAATCT NGG 10 8 17 8 24 0 100 5 10
    240606150216_ss7k GCAGAAAGCCTATGTCCTGCATGCTGGAACAG(+AAAAAA)GTACCTGTCTGTGTGTGGCCTTTCCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240606150214_wbq0 CCCAGATGTGGGGAAAGGCCACACACAGACAG(+AAAAAA)GTACCTGTTCCAGCATGCAGGACATAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606150211_a7wx CCCATTTCCGTTCACAGTATGTTGGGGCCCAG(+AAAAAA)GTACCAACAGTGTAGGGCTTTCTGATACCCT NGG 10 8 17 8 24 0 100 5 10
    240606150210_7ke5 TCCTGAGGGTATCAGAAAGCCCTACACTGTTG(+AAAAAA)GTACCTGGGCCCCAACATACTGTGAACGGAA NGG 10 8 17 8 24 0 100 5 10