Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150152_ooag AAACATTCCATGTTCCATTGTCGCTCCTCCGG(+AAAAAA)GTACCCAGTTGGGACCCTGGTGCCAGCATTT NGG 10 8 17 8 24 0 100 5 10
    240606150150_var5 CACTGAAATGCTGGCACCAGGGTCCCAACTGG(+AAAAAA)GTACCCGGAGGAGCGACAATGGAACATGGAA NGG 10 8 17 8 24 0 100 5 10
    240606150148_4bl2 CAGTCATGCCCAGCTTTCATAGGGGTTCTGGG(+AAAAAA)GATCCAAACTTAGGTCTTCACACATGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240606150147_sdyp ACGTGGTGAGCATGTGTGAAGACCTAAGTTTG(+AAAAAA)GATCCCCAGAACCCCTATGAAAGCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606150146_0u7o TGGACAGCCATGTTGCTATTTGGCATGAAGAG(+AAAAAA)GATCCCACTCAAAGATCCAAGGGAGGAATCT NGG 10 8 17 8 24 0 100 5 10
    240606150144_x9qi TCCCAAGATTCCTCCCTTGGATCTTTGAGTGG(+AAAAAA)GATCCTCTTCATGCCAAATAGCAACATGGCT NGG 10 8 17 8 24 0 100 5 10
    240606150143_v6y4 CAGATAGCCTTTGATGCATATAAAGGACAGCG(+AAAAAA)GATCCGCTGCACTCCAGGTGCTGCTCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606150142_23a9 TAGGAGGCTTGAGCAGCACCTGGAGTGCAGCG(+AAAAAA)GATCCGCTGTCCTTTATATGCATCAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606150140_3uzm GAAGAAGTTGATTTCAAACAAAAATTAAAAGG(+AAAAAA)GTACCCTGACTACTGGGGTGTGTGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606150138_v4sn CACACACACACACACACACCCCAGTAGTCAGG(+AAAAAA)GTACCCTTTTAATTTTTGTTTGAAATCAACT NGG 10 8 17 8 24 0 100 5 10