Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151508_xox8 TAATATGCCTTACATGGCACGCTCCAAGAATG(+AAAAAA)GTACCTGCCTACAACTCTTCCCATCCTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606151507_orfn CCATCTTGCTGACCCCTCCCCCATCTGGGCTG(+AAAAAA)GATCCAGCCATCTCGTCTTCCTCCTCACTGC NGG 10 8 17 8 24 0 100 5 10
    240606151505_95r3 TAGGGGCAGTGAGGAGGAAGACGAGATGGCTG(+AAAAAA)GATCCAGCCCAGATGGGGGAGGGGTCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606151503_67mi AAACCCAACTGTTCATCAATTCATCATCTTAG(+AAAAAA)GTACCAGCGATTAACCGACGTACTACAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606151502_t6dp GTACAAGCCTGTAGTACGTCGGTTAATCGCTG(+AAAAAA)GTACCTAAGATGATGAATTGATGAACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606151500_0aho AGGCATAGGCAATGTGAAGGTGAGGCCTGTGG(+AAAAAA)GTACCTAGTTCAGAACTCTGCTCAGCATCTG NGG 10 8 17 8 24 0 100 5 10
    240606151459_3i78 GCCTGCAGATGCTGAGCAGAGTTCTGAACTAG(+AAAAAA)GTACCCACAGGCCTCACCTTCACATTGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606151458_bzhz GTATATACACATATACATATAATTGATATCTG(+AAAAAA)GTACCACGTGACCCTCTGTGCTCCTCAACCC NGG 10 8 17 8 24 0 100 5 10
    240606151457_y6x8 CACCAGGGTTGAGGAGCACAGAGGGTCACGTG(+AAAAAA)GTACCAGATATCAATTATATGTATATGTGTA NGG 10 8 17 8 24 0 100 5 10
    240606151454_loie TGCAGCCATAGCAGGGCAACAGCAAGGCCTGG(+AAAAAA)GATCCCTTTGTCCAGACCCTGGGCAAGGCCA NGG 10 8 17 8 24 0 100 5 10