Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150407_eh4c CATGACAACAGTGGTGTCCACTCCCACATTCG(+AAAAAA)GATCCCAGGGTCCTACGTCCTCCTAAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606150404_wfwq CCCGACTGCTTTAGGAGGACGTAGGACCCTGG(+AAAAAA)GATCCGAATGTGGGAGTGGACACCACTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606150403_qh0m ATCGGCTGATGAGACCGGGTTCCATCTAAGCG(+AAAAAA)GTACCTGACTAACTCTGGCTCACTAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606150402_qejn GGGAACAGCCCTAGTGAGCCAGAGTTAGTCAG(+AAAAAA)GTACCGCTTAGATGGAACCCGGTCTCATCAG NGG 10 8 17 8 24 0 100 5 10
    240606150400_dk62 ACTAACTTGAGGTGAGGCAGCAAAAGGGCCTG(+AAAAAA)GTACCACCAAATGGTGGCTTCCACCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240606150359_p0n6 TGACCCCACGGGGTGGAAGCCACCATTTGGTG(+AAAAAA)GTACCAGGCCCTTTTGCTGCCTCACCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240606150357_mn16 AGACCAGCCTTCTCAGGTTCTCTGGCTCGTGG(+AAAAAA)GATCCTTCCAGGGAGCTACCTACAGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606150355_szsr TAACCCAAGCTCTGTAGGTAGCTCCCTGGAAG(+AAAAAA)GATCCCACGAGCCAGAGAACCTGAGAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606150354_q9qu CCAAGGAATGGAAGGCCCAGGTCTTTCTCCAG(+AAAAAA)GTACCGTACAGCTGGCCCCTTTGCTTACTGC NGG 10 8 17 8 24 0 100 5 10
    240606150352_02md GCAGAGCAGTAAGCAAAGGGGCCAGCTGTACG(+AAAAAA)GTACCTGGAGAAAGACCTGGGCCTTCCATTC NGG 10 8 17 8 24 0 100 5 10