Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150351_jv9i TCAATGGTGCTTTAAGGACGTGTGCAGGATAG(+AAAAAA)GTACCTTAGAGGAGCAGCCTAGTTTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606150350_ccig CATCAGCAGAGAAACTAGGCTGCTCCTCTAAG(+AAAAAA)GTACCTATCCTGCACACGTCCTTAAAGCACC NGG 10 8 17 8 24 0 100 5 10
    240606150349_bpu6 GTCCTGTTAGATGGAATCCTGTCATCTCATTG(+AAAAAA)GTACCCAGGGAGCCCAACTTCTTAGTAGCTC NGG 10 8 17 8 24 0 100 5 10
    240606150346_vwra CAAGAGAGCTACTAAGAAGTTGGGCTCCCTGG(+AAAAAA)GTACCAATGAGATGACAGGATTCCATCTAAC NGG 10 8 17 8 24 0 100 5 10
    240606150344_iqor TGGGTAACTACGGAGGCCAAAAGAAGGCATTG(+AAAAAA)GATCCCCTGGAGCTGTAGTTACAGGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606150342_v5t6 GCTCACAAGCACCTGTAACTACAGCTCCAGGG(+AAAAAA)GATCCAATGCCTTCTTTTGGCCTCCGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240606150340_k1xo GGCAGGTGAGTAGCTCTGAGCTCCTGCCTCAG(+AAAAAA)GTACCTTAACATGGACAGCAATGTGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240606150339_7p65 ATTCCAGTTTCCACATTGCTGTCCATGTTAAG(+AAAAAA)GTACCTGAGGCAGGAGCTCAGAGCTACTCAC NGG 10 8 17 8 24 0 100 5 10
    240606150338_2tz4 AGGTACTTCCTAAAGCTGCCCTGCCTTTACAG(+AAAAAA)GATCCCCCGAAAGAGAATTTAGGACGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606150335_h7my CAGGCTGCCTCGTCCTAAATTCTCTTTCGGGG(+AAAAAA)GATCCTGTAAAGGCAGGGCAGCTTTAGGAAG NGG 10 8 17 8 24 0 100 5 10