Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150453_omti GCCCACTGGAGGAATCCGGGGCTTGGCAGTGG(+AAAAAA)GATCCTGCTGAACTGTACTTCGAATACATCC NGG 10 8 17 8 24 0 100 5 10
    240606150452_ahd3 AATTTGGATGTATTCGAAGTACAGTTCAGCAG(+AAAAAA)GATCCCACTGCCAAGCCCCGGATTCCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606150450_6gdu TCCCTCCGTTCTGGGCTGTTAGCACCACTCAG(+AAAAAA)GTACCAAGGACTGCTCTGAGCACAGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606150448_qb7o AAGTCTCTCTACTGTGCTCAGAGCAGTCCTTG(+AAAAAA)GTACCTGAGTGGTGCTAACAGCCCAGAACGG NGG 10 8 17 8 24 0 100 5 10
    240606150447_xp6x AGCCAACAGGAAAAACTACTGAAAATGTCATG(+AAAAAA)GATCCCATGTCAGTCTGGCACTTGATTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606150446_h7ib GGCACAAAGAATCAAGTGCCAGACTGACATGG(+AAAAAA)GATCCATGACATTTTCAGTAGTTTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606150444_p8k9 CACTAACCACAACACAGTACAAGTGTCTCTGG(+AAAAAA)GTACCCCGAACATCTCCCCGAAGAGTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240606150442_79q9 GAGGACTAGAACTCTTCGGGGAGATGTTCGGG(+AAAAAA)GTACCCAGAGACACTTGTACTGTGTTGTGGT NGG 10 8 17 8 24 0 100 5 10
    240606150441_ypem ATATTCATGTTTATTCGCTCTCTACCAAGTAG(+AAAAAA)GATCCATTTTTGATAAAGCAGTCTTTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606150440_gw4d GAAGAAACCCAAAGACTGCTTTATCAAAAATG(+AAAAAA)GATCCTACTTGGTAGAGAGCGAATAAACATG NGG 10 8 17 8 24 0 100 5 10