Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151742_ow5w TGAGGGCAGGATGCCAGGAGCTGGCTGTGCCG(+AAAAAA)GATCCCGCCTGCACTAATAACTTCTATTACA NGG 10 8 17 8 24 0 100 5 10
    240606151741_rlq9 AGATACCTCCGTTTTCTTTGTGACCGGCGTTG(+AAAAAA)GATCCTGGATAGTTATGGCTGGCTGCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606151739_i40w CGCAGTGACTGCAGCCAGCCATAACTATCCAG(+AAAAAA)GATCCAACGCCGGTCACAAAGAAAACGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606151737_3u3q CACCAGCCCAGCTCCTGTCCACCGCTGCTCGG(+AAAAAA)GTACCCAGGGCTACCCCAGACCCTGCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606151734_mwmm GAGCCTCTCTGCAGGGTCTGGGGTAGCCCTGG(+AAAAAA)GTACCCGAGCAGCGGTGGACAGGAGCTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606151733_169s GGCCTCAGTGCTCAGCCTGCTCTTTAATAAAG(+AAAAAA)GTACCTCTTTTCTGAGCCATGTCGGAATGAG NGG 10 8 17 8 24 0 100 5 10
    240606151731_zq9x CTCCACTCATTCCGACATGGCTCAGAAAAGAG(+AAAAAA)GTACCTTTATTAAAGAGCAGGCTGAGCACTG NGG 10 8 17 8 24 0 100 5 10
    240606151730_0e1t CCTTACCCTGTACTAGGCCCATCAGTCACAGG(+AAAAAA)GTACCTGGGGCCACTATGCACCATGGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606151727_z0t5 AGTAAACTGACCATGGTGCATAGTGGCCCCAG(+AAAAAA)GTACCCTGTGACTGATGGGCCTAGTACAGGG NGG 10 8 17 8 24 0 100 5 10
    240606151725_3g0v TCCCTGCCCTCCCCTGTCCCGGCCCAGCCTGG(+AAAAAA)GATCCATGGGAGTGGGAGGGGTAACTTGAGG NGG 10 8 17 8 24 0 100 5 10