Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151848_wq33 GGTAATCCTGTATTTCAGGGAAGTAGGAGGTG(+AAAAAA)GTACCTAAGGATGACTTATGAAGGATTGTAG NGG 10 8 17 8 24 0 100 5 10
    240606151846_ufqn GCTAACTGACCAAGCTTCTCCCGCTCGTGCAG(+AAAAAA)GTACCTCCCTGATGGGAACACCAGAGACTGC NGG 10 8 17 8 24 0 100 5 10
    240606151844_z1w5 GAGCAGCAGTCTCTGGTGTTCCCATCAGGGAG(+AAAAAA)GTACCTGCACGAGCGGGAGAAGCTTGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606151843_7cx7 GGAAGAAAGACTGAGACCATCTGCTTTGGATG(+AAAAAA)GATCCATTAGCAAGCCTGGTCTGACTACACA NGG 10 8 17 8 24 0 100 5 10
    240606151842_6ucy GCCCCTGTGTAGTCAGACCAGGCTTGCTAATG(+AAAAAA)GATCCATCCAAAGCAGATGGTCTCAGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606151840_3v88 CTTTGTGGTAAGTCTTTGCTTCAGACTGTTGG(+AAAAAA)GTACCCAGAACGAGAGCTATAAAAATGCATT NGG 10 8 17 8 24 0 100 5 10
    240606151838_7uil TGAACAATGCATTTTTATAGCTCTCGTTCTGG(+AAAAAA)GTACCCAACAGTCTGAAGCAAAGACTTACCA NGG 10 8 17 8 24 0 100 5 10
    240606151836_j2rl CTTTATTTGGATAGCAGCGCTTCAGTTCCTAG(+AAAAAA)GATCCTTTTATAAATGGTAAAACCCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240606151835_i7qv TTGTAAATCCAGGGTTTTACCATTTATAAAAG(+AAAAAA)GATCCTAGGAACTGAAGCGCTGCTATCCAAA NGG 10 8 17 8 24 0 100 5 10
    240606151834_u8mu CTGACAAAGAGGTGGGCCAGGATGGGACATGG(+AAAAAA)GTACCAGGAGTGCACAGGCTTGGGCAATTTG NGG 10 8 17 8 24 0 100 5 10