Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150711_ppop TGGGATGTGGAGAGAAGGTATTCTATGAGTGG(+AAAAAA)GTACCACTGTTAGGACATGGGGGTCGGGGCG NGG 10 8 17 8 24 0 100 5 10
    240606150710_kymc TTTACCGCCCCGACCCCCATGTCCTAACAGTG(+AAAAAA)GTACCCACTCATAGAATACCTTCTCTCCACA NGG 10 8 17 8 24 0 100 5 10
    240606150708_52qo GGGATTATATGATGGGCTGGAGGAGTTTGGAG(+AAAAAA)GATCCCCATTCAGAGTGGGGCCTAGGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606150706_pk4p ACAACCAGGCCCTAGGCCCCACTCTGAATGGG(+AAAAAA)GATCCTCCAAACTCCTCCAGCCCATCATATA NGG 10 8 17 8 24 0 100 5 10
    240606150705_72wj TAGTAATAGAACAGTGATTAAGACAAAAGTTG(+AAAAAA)GTACCAAGGAGCAAAGTATTCTATGAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606150703_yvk0 GTTAGGCTTTTCATAGAATACTTTGCTCCTTG(+AAAAAA)GTACCAACTTTTGTCTTAATCACTGTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240606150701_xiw5 ATGGATTTCTTACTCCTCTTCTCCATGGCTGG(+AAAAAA)GATCCTAGCTTCAGTCCTCATATCTGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240606150700_l57f GGTGAGTAAACAGATATGAGGACTGAAGCTAG(+AAAAAA)GATCCCAGCCATGGAGAAGAGGAGTAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606150659_8vh1 TGCACACATGACATCTATGTAGCATATGCTAG(+AAAAAA)GATCCAGACGGACCTTAGAGTCTAAATCACC NGG 10 8 17 8 24 0 100 5 10
    240606150657_5k8j AGCATGGTGATTTAGACTCTAAGGTCCGTCTG(+AAAAAA)GATCCTAGCATATGCTACATAGATGTCATGT NGG 10 8 17 8 24 0 100 5 10