Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150656_mwck AGCACTTCACCTGCACGAAGGGATGAGACTGG(+AAAAAA)GATCCCTGAGTAAACTTCTTTAATAGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606150655_ee0h ATAGCACACACTATTAAAGAAGTTTACTCAGG(+AAAAAA)GATCCCAGTCTCATCCCTTCGTGCAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606150653_72x4 CTGGTTCCCATGGAAGCCAGAAGAGGGTGCTG(+AAAAAA)GATCCCTTGGAACTTGAGTTACAGACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606150651_x71z GGTTGCAGCTGTCTGTAACTCAAGTTCCAAGG(+AAAAAA)GATCCAGCACCCTCTTCTGGCTTCCATGGGA NGG 10 8 17 8 24 0 100 5 10
    240606150650_2q71 CTTTCATATTGCCACTAGGTCAGCCATTAAAG(+AAAAAA)GTACCACAAAATCACCTGTTTATGTGAATTA NGG 10 8 17 8 24 0 100 5 10
    240606150649_dndh TTCATTAATTCACATAAACAGGTGATTTTGTG(+AAAAAA)GTACCTTTAATGGCTGACCTAGTGGCAATAT NGG 10 8 17 8 24 0 100 5 10
    240606150648_qft4 TTTCAGCCCCTTGTATCACTCTTCCTGATCTG(+AAAAAA)GTACCTGAGCAACCGCTGATCTGCTTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606150646_d5ta TCAAGAGAAAAAGCAGATCAGCGGTTGCTCAG(+AAAAAA)GTACCAGATCAGGAAGAGTGATACAAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606150644_p3l3 GGCCTTGCTTCACCACCCAAGCCAGCTGCTGG(+AAAAAA)GTACCCAGCCTGACTGCTGAAGTAAGACACT NGG 10 8 17 8 24 0 100 5 10
    240606150643_6h18 GCTGCAGTGTCTTACTTCAGCAGTCAGGCTGG(+AAAAAA)GTACCCAGCAGCTGGCTTGGGTGGTGAAGCA NGG 10 8 17 8 24 0 100 5 10