Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150744_3pxt AGTTTAGTCACAGCGTAGGGGTGCTTCTGTGG(+AAAAAA)GTACCCAGCCTCAGCTGTGATCCTAAGAGTA NGG 10 8 17 8 24 0 100 5 10
    240606150743_m7lm CACCCTACTCTTAGGATCACAGCTGAGGCTGG(+AAAAAA)GTACCCACAGAAGCACCCCTACGCTGTGACT NGG 10 8 17 8 24 0 100 5 10
    240606150740_98v2 AGCCAGCATGAATTCGTGTCCAGTTTTCCTGG(+AAAAAA)GATCCAAATTCTCAAGGTTATTTCTGAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606150739_h1q6 AAGAACAACTCAGAAATAACCTTGAGAATTTG(+AAAAAA)GATCCCAGGAAAACTGGACACGAATTCATGC NGG 10 8 17 8 24 0 100 5 10
    240606150737_ovh1 ATGAGCTGAGAGGCCCTGCAGGGCCCTCCATG(+AAAAAA)GATCCATGCAGTTGGAGATGCTAATGCCTAT NGG 10 8 17 8 24 0 100 5 10
    240606150736_onfd TCCTGATAGGCATTAGCATCTCCAACTGCATG(+AAAAAA)GATCCATGGAGGGCCCTGCAGGGCCTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606150734_kpha ACTAAGACACTTTCCCTCAGCGTGTGACTCAG(+AAAAAA)GATCCTCTGTGTTTGAATTTCACATGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606150733_ae1q ACCCTTCTCCCATGTGAAATTCAAACACAGAG(+AAAAAA)GATCCTGAGTCACACGCTGAGGGAAAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606150730_38f8 CATACCTGAAATGTAAGTGAAGTCAGCCCATG(+AAAAAA)GATCCCAGAGCAGCCTCACTGTGCATGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606150729_6f90 AGATCCCACCATGCACAGTGAGGCTGCTCTGG(+AAAAAA)GATCCATGGGCTGACTTCACTTACATTTCAG NGG 10 8 17 8 24 0 100 5 10