Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150816_luci ATATAGTATAAATGTTTTTATGTACCAGTGAG(+AAAAAA)GTACCAGTGTCTCTCTGGAGGTTGGATCTGT NGG 10 8 17 8 24 0 100 5 10
    240606150814_7mri AGACCACAGATCCAACCTCCAGAGAGACACTG(+AAAAAA)GTACCTCACTGGTACATAAAAACATTTATAC NGG 10 8 17 8 24 0 100 5 10
    240606150813_8x1z CACCATACCTGTTTTATGTGTGTCTCCTTCTG(+AAAAAA)GATCCTCTCTCGGTGTGGTTGCCACTACCAC NGG 10 8 17 8 24 0 100 5 10
    240606150812_1t6t TCCAAGTGGTAGTGGCAACCACACCGAGAGAG(+AAAAAA)GATCCAGAAGGAGACACACATAAAACAGGTA NGG 10 8 17 8 24 0 100 5 10
    240606150810_bjao TTATATATTGTCCATTAAACCCATGTTCCATG(+AAAAAA)GTACCTCACTGGAAGAATTGGGTCCTTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606150809_hp0e CCTTTAAGAAAGGACCCAATTCTTCCAGTGAG(+AAAAAA)GTACCATGGAACATGGGTTTAATGGACAATA NGG 10 8 17 8 24 0 100 5 10
    240606150807_353q AGAGCACACACACACAGCTCGTGTGCGTGCGG(+AAAAAA)GATCCTCTTCTACTCTCATCATCACATACTG NGG 10 8 17 8 24 0 100 5 10
    240606150806_p5cx GAACACAGTATGTGATGATGAGAGTAGAAGAG(+AAAAAA)GATCCCGCACGCACACGAGCTGTGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606150803_5hpw GAGGACTTCCAGGAAGCCATATGGCTGCCCTG(+AAAAAA)GTACCAAGGAGTTGAGTTTACCATGAATACC NGG 10 8 17 8 24 0 100 5 10
    240606150801_u2a1 GCTGCGGTATTCATGGTAAACTCAACTCCTTG(+AAAAAA)GTACCAGGGCAGCCATATGGCTTCCTGGAAG NGG 10 8 17 8 24 0 100 5 10