Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150916_4ld8 CACACCTCACAGAGCATGGAGAAGTCAAACTG(+AAAAAA)GTACCTACCAGAGCCTTCACCTCTCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240606150915_hijb AGGTAGTCCATGAGAGGTGAAGGCTCTGGTAG(+AAAAAA)GTACCAGTTTGACTTCTCCATGCTCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606150913_kr57 TAGAGTATTTGTAACTGTATTCTCTAGCTGGG(+AAAAAA)GATCCATTGTTTTTATATTTCAAGTACACTG NGG 10 8 17 8 24 0 100 5 10
    240606150911_twtd AAATACAGTGTACTTGAAATATAAAAACAATG(+AAAAAA)GATCCCCAGCTAGAGAATACAGTTACAAATA NGG 10 8 17 8 24 0 100 5 10
    240606150910_yf92 GCTCTCCATCACTGCTCATCACAGAAAGGAAG(+AAAAAA)GTACCTGGCCTGAGGTCAGGGCCATCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606150908_yg49 CAGACAGAGGGATGGCCCTGACCTCAGGCCAG(+AAAAAA)GTACCTTCCTTTCTGTGATGAGCAGTGATGG NGG 10 8 17 8 24 0 100 5 10
    240606150906_upeb ATTGGAAACCAACTGTAACTCCAGTTCCACAG(+AAAAAA)GATCCGATGCCCTCCTCTGGCCTCTGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240606150905_6uj0 CTGGTGCTTGCAGAGGCCAGAGGAGGGCATCG(+AAAAAA)GATCCTGTGGAACTGGAGTTACAGTTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606150903_bnk5 ATTGGAAACCAACTGTAACTCCAGTTCCACAG(+AAAAAA)GATCCGATGCCCTCCTCTGGCCTCTGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240606150902_hc5c CTGGTGCTTGCAGAGGCCAGAGGAGGGCATCG(+AAAAAA)GATCCTGTGGAACTGGAGTTACAGTTGGTTT NGG 10 8 17 8 24 0 100 5 10