Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151002_haxi GAAAGAATTGGCATGTTTTTTAATTTGCAGGG(+AAAAAA)GATCCTTGTGAGCATCTAGATAGTCCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606151001_40mb AAGAGAGAAGGGACTATCTAGATGCTCACAAG(+AAAAAA)GATCCCCTGCAAATTAAAAAACATGCCAATT NGG 10 8 17 8 24 0 100 5 10
    240606150959_wfu8 CCAGGGCTTTAGGATTGCTGAGTGCTCCGGAG(+AAAAAA)GATCCTCACATACCCACTGTTACTGTTCACT NGG 10 8 17 8 24 0 100 5 10
    240606150957_kj4b TCTAAAGTGAACAGTAACAGTGGGTATGTGAG(+AAAAAA)GATCCTCCGGAGCACTCAGCAATCCTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606150956_sjvk TCCAGGGTATCTAACACTTCTGGCCTCCACAG(+AAAAAA)GTACCTGTCTCACAGGACCATAACCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240606150954_y535 TTTCTCTGTGGGGTTATGGTCCTGTGAGACAG(+AAAAAA)GTACCTGTGGAGGCCAGAAGTGTTAGATACC NGG 10 8 17 8 24 0 100 5 10
    240606150953_2alz TTGTGACAAATATTTTTGCCTTTCAAAGTTAG(+AAAAAA)GTACCAGAAATATCAAGACTTGGGTTAACCA NGG 10 8 17 8 24 0 100 5 10
    240606150952_ir4g TATCATGGTTAACCCAAGTCTTGATATTTCTG(+AAAAAA)GTACCTAACTTTGAAAGGCAAAAATATTTGT NGG 10 8 17 8 24 0 100 5 10
    240606150950_6f1s AATCCACAGAGGATTTCACATGATGTCAGCCG(+AAAAAA)GTACCGGGAGGAGCCATGCAGGATGGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240606150948_18sc GACAAGTGCACCATCCTGCATGGCTCCTCCCG(+AAAAAA)GTACCGGCTGACATCATGTGAAATCCTCTGT NGG 10 8 17 8 24 0 100 5 10