Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151017_9mqf AGGTCCTCCTGCTTCAGCTTCTTACACAGAGG(+AAAAAA)GATCCCAGAGTGTGCCACCACCGCCAGCGTC NGG 10 8 17 8 24 0 100 5 10
    240606151015_vmg8 GCGGTGACGCTGGCGGTGGTGGCACACTCTGG(+AAAAAA)GATCCCTCTGTGTAAGAAGCTGAAGCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606151013_hsyl CTTCCAGCAGGAGACCCCGCCCTCACTGGGTG(+AAAAAA)GATCCTTCTTTAGTATCACGTGCTCACACTG NGG 10 8 17 8 24 0 100 5 10
    240606151012_aja4 CAAGCCAGTGTGAGCACGTGATACTAAAGAAG(+AAAAAA)GATCCACCCAGTGAGGGCGGGGTCTCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606151011_ty6s CTATACCTAGACCACAGTTTGATCTGTAGCTG(+AAAAAA)GTACCTCATCAGAGCATAGCTCCGCCATCCC NGG 10 8 17 8 24 0 100 5 10
    240606151010_y9dj CAGCAGGGATGGCGGAGCTATGCTCTGATGAG(+AAAAAA)GTACCAGCTACAGATCAAACTGTGGTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240606151009_xqe9 GACCACCCCAGGTCTTGTGTGTTTTCTATAAG(+AAAAAA)GTACCAGAATAAATGGGAAGTTGATTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606151007_gakd ATTTGTTTTAAATCAACTTCCCATTTATTCTG(+AAAAAA)GTACCTTATAGAAAACACACAAGACCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606151006_3g18 AGAAAGCGTGCGCTATTCTGACTAATGCCTTG(+AAAAAA)GATCCCTCATCCTACCCATGTTTACTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606151004_8d9b CAAATACAGAAGTAAACATGGGTAGGATGAGG(+AAAAAA)GATCCAAGGCATTAGTCAGAATAGCGCACGC NGG 10 8 17 8 24 0 100 5 10