Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151118_ggrs GTTCTGTAAACAGTAAGTGAGGTCGTCTCTAG(+AAAAAA)GATCCTGAAGAGTCACACATGCACGCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240606151117_6em6 AAATGTGCAAGCGTGCATGTGTGACTCTTCAG(+AAAAAA)GATCCTAGAGACGACCTCACTTACTGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240606151115_w0pv GAATTCCTTTAGTGACAAGCATCTCTCAGAGG(+AAAAAA)GTACCTCTGATGCACTAATGTATACCTACCC NGG 10 8 17 8 24 0 100 5 10
    240606151114_732b TTTCTGGGTAGGTATACATTAGTGCATCAGAG(+AAAAAA)GTACCCTCTGAGAGATGCTTGTCACTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606151113_clgc CATGAGCAAACTTTTGAAACATCTTCAAGAAG(+AAAAAA)GTACCAAGGGATTGCAGTGCATAATTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606151110_6dzk TTACAGCCAAAATTATGCACTGCAATCCCTTG(+AAAAAA)GTACCTTCTTGAAGATGTTTCAAAAGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240606151109_pwwg TCTGACCCACATCAAGCCTCAGACGACAGATG(+AAAAAA)GTACCTGCCTGTTAAAGCATAACAGGGTGAT NGG 10 8 17 8 24 0 100 5 10
    240606151107_3d75 CACCCATCACCCTGTTATGCTTTAACAGGCAG(+AAAAAA)GTACCATCTGTCGTCTGAGGCTTGATGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606151106_6zbi ACTCCCCACTGGCATAAGGTGCCGGTCTGTGG(+AAAAAA)GTACCATCCGAGCTCCTGAGCAATGCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606151104_jgv2 TTGGGACTGAGCATTGCTCAGGAGCTCGGATG(+AAAAAA)GTACCCACAGACCGGCACCTTATGCCAGTGG NGG 10 8 17 8 24 0 100 5 10