Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151132_hjme TGGTTGCTTATTTCTCCCATGGAGAAACAAAG(+AAAAAA)GATCCTACTGGAACATTGTCCTCACACCTGA NGG 10 8 17 8 24 0 100 5 10
    240606151131_et92 GACCATCAGGTGTGAGGACAATGTTCCAGTAG(+AAAAAA)GATCCTTTGTTTCTCCATGGGAGAAATAAGC NGG 10 8 17 8 24 0 100 5 10
    240606151129_qz4m AGATCTTAACTTACAAATAATTTATAGGGATG(+AAAAAA)GATCCACTCCCTCATAATTTGATCTGCAACA NGG 10 8 17 8 24 0 100 5 10
    240606151128_wzsu TGATCTGTTGCAGATCAAATTATGAGGGAGTG(+AAAAAA)GATCCATCCCTATAAATTATTTGTAAGTTAA NGG 10 8 17 8 24 0 100 5 10
    240606151126_fjmi AATTGCTTAGGTTAGTTCATCTGGAACACAGG(+AAAAAA)GTACCACTATGAAGCTCAATTTCACTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606151125_j2hm TATCTGCTCAAGTGAAATTGAGCTTCATAGTG(+AAAAAA)GTACCCTGTGTTCCAGATGAACTAACCTAAG NGG 10 8 17 8 24 0 100 5 10
    240606151124_rea7 AACATTTATACAAACCCTGTAACTCCATCAAG(+AAAAAA)GATCCATTTGAATGGTATTGAAGTCAGCCAA NGG 10 8 17 8 24 0 100 5 10
    240606151122_0j3x GTAGCTTGGCTGACTTCAATACCATTCAAATG(+AAAAAA)GATCCTTGATGGAGTTACAGGGTTTGTATAA NGG 10 8 17 8 24 0 100 5 10
    240606151121_l9aq TTAGAGAAGGTGGTCCCTCAGGATGCAGCTGG(+AAAAAA)GATCCACGCAGGCCCCAGGACACTAGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606151120_dyxr GTAGGGCAGCCTAGTGTCCTGGGGCCTGCGTG(+AAAAAA)GATCCCAGCTGCATCCTGAGGGACCACCTTC NGG 10 8 17 8 24 0 100 5 10