Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151236_fng6 AGTCTTTTTTACACTCTGCCTGTGGTCCCAGG(+AAAAAA)GATCCTGGCTCAGAGGACTCAAGCGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606151235_l0v5 CCTGTCCCCTGCGCTTGAGTCCTCTGAGCCAG(+AAAAAA)GATCCCTGGGACCACAGGCAGAGTGTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606151234_4rh3 CTTGTGGGGCTTTTAGTTTGAAGACCCTGAAG(+AAAAAA)GATCCCACTGCATAGTGAGTCTGTCCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606151232_hv6x TTAATAACCAGGACAGACTCACTATGCAGTGG(+AAAAAA)GATCCTTCAGGGTCTTCAAACTAAAAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606151230_3n4q AAAGTCTCTGTGCTCTACTGCCAAGAGGCCGG(+AAAAAA)GATCCTCTGGCCCACTGTTTCCCCAGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240606151228_g2zb CCAGAGTGAGCTGGGGAAACAGTGGGCCAGAG(+AAAAAA)GATCCCGGCCTCTTGGCAGTAGAGCACAGAG NGG 10 8 17 8 24 0 100 5 10
    240606151227_jrr0 TCCCAAATACTTCTTCCACTTCCACATTAGAG(+AAAAAA)GATCCACAAATCAAAATATCATCCATACTAC NGG 10 8 17 8 24 0 100 5 10
    240606151225_acrr AAAGCGTAGTATGGATGATATTTTGATTTGTG(+AAAAAA)GATCCTCTAATGTGGAAGTGGAAGAAGTATT NGG 10 8 17 8 24 0 100 5 10
    240606151224_1gcc ATACTGGGGTGCTTTAGGGGTCCAGAGTACTG(+AAAAAA)GTACCACTGTCCTATGGCCTGGGATGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606151223_qkl2 TGGAACCTGACATCCCAGGCCATAGGACAGTG(+AAAAAA)GTACCAGTACTCTGGACCCCTAAAGCACCCC NGG 10 8 17 8 24 0 100 5 10