Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151313_xc97 CACTCCTCCTAGGGGAAAGCTGAACCCTGTGG(+AAAAAA)GATCCCGGGAACAGGGATAGCCCAGCTCCGG NGG 10 8 17 8 24 0 100 5 10
    240606151310_z6o3 CAGAACCGGAGCTGGGCTATCCCTGTTCCCGG(+AAAAAA)GATCCCACAGGGTTCAGCTTTCCCCTAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606151309_cyh4 GCCACGTGGTGGCTCACAACCATCTGTAACAG(+AAAAAA)GATCCGATGCCTTCTTCTGGTGCATCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606151308_jqct GCTGTCTTCAGATGCACCAGAAGAAGGCATCG(+AAAAAA)GATCCTGTTACAGATGGTTGTGAGCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240606151306_b8rw GCCCCCAATGTCTGCTGTGCGCAGCAGCAAGG(+AAAAAA)GTACCGATGAGAAGCCTAGTTAAGCCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606151304_v1i5 CCTCTCACAGGGCTTAACTAGGCTTCTCATCG(+AAAAAA)GTACCCTTGCTGCTGCGCACAGCAGACATTG NGG 10 8 17 8 24 0 100 5 10
    240606151303_e1e2 GAAGCCTAGTTAAGCCCTGTGAGAGGGCTAGG(+AAAAAA)GATCCAAGTAGTGAAAGCCAGAAGAGGCGAG NGG 10 8 17 8 24 0 100 5 10
    240606151302_jrxn AGCATCTCGCCTCTTCTGGCTTTCACTACTTG(+AAAAAA)GATCCCTAGCCCTCTCACAGGGCTTAACTAG NGG 10 8 17 8 24 0 100 5 10
    240606151300_pr3q CAGGGGCTATCAGGCTGAGAGATGCCAAACCG(+AAAAAA)GTACCCGCCAAAGGACTAGAACCCCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606151258_2pf3 TGTTCCTTCTGGGGGTTCTAGTCCTTTGGCGG(+AAAAAA)GTACCGGTTTGGCATCTCTCAGCCTGATAGC NGG 10 8 17 8 24 0 100 5 10