Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152617_zuz1 GAGAGAGGCGGGGGGAGCAGAGGCTTCCGTTG(+AAAAAA)GATCCTAGTAGAGGACAGCGGGGGGCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240606152615_2cwe TGAAAGGACTCTTTTCTGCCCAAGGGACACAG(+AAAAAA)GATCCGTCAGTTCCACTCCCTGCATGCACCA NGG 10 8 17 8 24 0 100 5 10
    240606152614_zatf GGTCGTGGTGCATGCAGGGAGTGGAACTGACG(+AAAAAA)GATCCTGTGTCCCTTGGGCAGAAAAGAGTCC NGG 10 8 17 8 24 0 100 5 10
    240606152611_rmmi TTGGCCACTTGACTTTGGTCCAGTGATCCCGG(+AAAAAA)GTACCGGTAACGGGCATAAGTTTCAGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606152607_mf4y GACCCAGGAGCTGAAACTTATGCCCGTTACCG(+AAAAAA)GTACCCGGGATCACTGGACCAAAGTCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606152606_96rt GGGGTATGTGGGAGCCGGTACACACGGTGCTG(+AAAAAA)GATCCAGAGCTGGGGTGGGGTGCATGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606152605_rnze ACCCCAGGGACATGCACCCCACCCCAGCTCTG(+AAAAAA)GATCCAGCACCGTGTGTACCGGCTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240606152603_yuvc GGGGTATGTGGGAGCCGGTACACACGGTGCTG(+AAAAAA)GATCCAGAGCTGGGGTGGGGTGCATGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606152602_0pe0 ACCCCAGGGACATGCACCCCACCCCAGCTCTG(+AAAAAA)GATCCAGCACCGTGTGTACCGGCTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240606152559_iu6g GAACGCCTACTTCCTGTCTCTGCTGTCTCCCG(+AAAAAA)GTACCTGTGCTTCTGAGCCCAGACTATCTCT NGG 10 8 17 8 24 0 100 5 10