Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151344_wyi9 GGAACCAGGGATTCTGTAGCAGACATGGGCTG(+AAAAAA)GATCCATACACGGTCCCTGTGAGTTCATTTC NGG 10 8 17 8 24 0 100 5 10
    240606151343_a5vv ATTGAGAAATGAACTCACAGGGACCGTGTATG(+AAAAAA)GATCCAGCCCATGTCTGCTACAGAATCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606151342_0z1n AACTGAATGGCTAATTTATTGAGAACCTATTG(+AAAAAA)GATCCACTCACAGGATTGAAGGACGCCCTCA NGG 10 8 17 8 24 0 100 5 10
    240606151340_w067 CTCATTGAGGGCGTCCTTCAATCCTGTGAGTG(+AAAAAA)GATCCAATAGGTTCTCAATAAATTAGCCATT NGG 10 8 17 8 24 0 100 5 10
    240606151339_ftkz CACACCCACCTCCATCCCGAGTGCCTGGATGG(+AAAAAA)GTACCGAGCTTAATGGACACCTTTGAAATAG NGG 10 8 17 8 24 0 100 5 10
    240606151337_lhdw CAGGTCTATTTCAAAGGTGTCCATTAAGCTCG(+AAAAAA)GTACCCATCCAGGCACTCGGGATGGAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606151335_pp92 TATTCTTCTAAAGGGCTGTGAAATGCCCTAAG(+AAAAAA)GTACCCATACCTCAATTGAAATGGCATTCTC NGG 10 8 17 8 24 0 100 5 10
    240606151334_uysq AGCCAGAGAATGCCATTTCAATTGAGGTATGG(+AAAAAA)GTACCTTAGGGCATTTCACAGCCCTTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240606151333_cz50 TGATAATGCACACTTTCTCTAAAGGGGAGCTG(+AAAAAA)GATCCCACAGTTGGACAGTTTTCACTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606151331_0hni GTACATTTTAAGTGAAAACTGTCCAACTGTGG(+AAAAAA)GATCCAGCTCCCCTTTAGAGAAAGTGTGCAT NGG 10 8 17 8 24 0 100 5 10