Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151329_rv5m CCCATCCTGCACAGGGAGCTGGATGCTCTTGG(+AAAAAA)GATCCATACACATGTGGATCTGAGTTAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606151328_byog ACAGAACACTAACTCAGATCCACATGTGTATG(+AAAAAA)GATCCCAAGAGCATCCAGCTCCCTGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240606151326_gkxf CCTTAATAATGGCCCTGATGTACTGCAAGTAG(+AAAAAA)GATCCTGTCTCCAACCCCAGCTCAGACAGTA NGG 10 8 17 8 24 0 100 5 10
    240606151325_bxhc GGCTGTACTGTCTGAGCTGGGGTTGGAGACAG(+AAAAAA)GATCCTACTTGCAGTACATCAGGGCCATTAT NGG 10 8 17 8 24 0 100 5 10
    240606151324_vqix AGGGATGCACTCTGGGCTCCAGAAGCAGGCAG(+AAAAAA)GTACCCGAGTCTATGCTAGGACCTTAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606151322_d2w0 TTATGGTGCTTAAGGTCCTAGCATAGACTCGG(+AAAAAA)GTACCTGCCTGCTTCTGGAGCCCAGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240606151320_2dl6 CAGAGGAGACCCTACGAACGCCTAAGAGCCAG(+AAAAAA)GATCCTCCCAGCCCCCAGCCCGCCACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606151318_kc28 TGCTGGAAGGGTGGCGGGCTGGGGGCTGGGAG(+AAAAAA)GATCCTGGCTCTTAGGCGTTCGTAGGGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606151316_wt02 CTATGGCACGTTTCTGTTACCCAATGCCAAGG(+AAAAAA)GTACCATCTTGCCACCTTGTCTCTCAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606151315_hn3l TCTCTATTCTTGAGAGACAAGGTGGCAAGATG(+AAAAAA)GTACCCTTGGCATTGGGTAACAGAAACGTGC NGG 10 8 17 8 24 0 100 5 10