Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152647_iiia CATTTTTTACTTCTGTCTGCTCTATTTCACTG(+AAAAAA)GATCCTTATAATAATCCTGAATGATTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606152646_qcg4 CTCCATCCTTGCACTCGCCACTACCCTAGAAG(+AAAAAA)GATCCTTCTGCTGCCCTCTGCCTCCCATCCG NGG 10 8 17 8 24 0 100 5 10
    240606152645_19l1 TGGGGCGGATGGGAGGCAGAGGGCAGCAGAAG(+AAAAAA)GATCCTTCTAGGGTAGTGGCGAGTGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606152643_1qhb TATTTTGGAATAAAATTTATTTTATGGCTTAG(+AAAAAA)GATCCATGGCCCCCTTCCCCTCCAGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240606152642_81ym TTTTTCCCTCACTGGAGGGGAAGGGGGCCATG(+AAAAAA)GATCCTAAGCCATAAAATAAATTTTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240606152640_4frd TATCAGCCAGTGTGGCCCTCTATTTCCTTGTG(+AAAAAA)GTACCGAGTGTCCCTGAGGCTGGGGTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606152639_bxim GAAAAAGACCACCCCAGCCTCAGGGACACTCG(+AAAAAA)GTACCACAAGGAAATAGAGGGCCACACTGGC NGG 10 8 17 8 24 0 100 5 10
    240606152637_2cnf AGCATGCAGACATCCCTCTGTGGACTTCAGGG(+AAAAAA)GATCCACCTCTAAAGAGAGTCACTAGTGACC NGG 10 8 17 8 24 0 100 5 10
    240606152636_xjvt CTGTTGGTCACTAGTGACTCTCTTTAGAGGTG(+AAAAAA)GATCCCCTGAAGTCCACAGAGGGATGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606152634_md79 GGGTTCCCACGTGTCTGGAAGCTCGAGTTCCG(+AAAAAA)GATCCATACTGACACCCAATAAGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10