Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151417_2wld CAAAGTGCCTTTGTCTGCCTGGAGTCAACTTG(+AAAAAA)GATCCATTGGAAACATAGAAAGGGTCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606151415_w4kg CTGTCCCCTGGACCCTTTCTATGTTTCCAATG(+AAAAAA)GATCCAAGTTGACTCCAGGCAGACAAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606151413_d7bj CAGGTGGCTGTGGGGGACAGAAGAGGGTGCCG(+AAAAAA)GATCCCCTGGAGCTGGAGTTAGGCTTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240606151411_5qyb TTAGTAACTAAAGCCTAACTCCAGCTCCAGGG(+AAAAAA)GATCCGGCACCCTCTTCTGTCCCCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240606151410_qxzo AGCAGAGCCCTGGCCCTATTAGCAGCACAGTG(+AAAAAA)GTACCCGAGGGTCTCTGTCAACAAGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240606151407_dr9l TCATTTCCTGTCTTGTTGACAGAGACCCTCGG(+AAAAAA)GTACCACTGTGCTGCTAATAGGGCCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606151406_avfj CACACACAGCACCCATGCTAGCACGCACAGAG(+AAAAAA)GATCCCGTTGATCACTCCAGAAGAGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240606151404_ljqj ACCCAGGTCACCTCTTCTGGAGTGATCAACGG(+AAAAAA)GATCCTCTGTGCGTGCTAGCATGGGTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606151402_ka92 AATTAAATGTTGTCCTTATAAGAGTTGCCTTG(+AAAAAA)GTACCAGGAGAGCCAGGGCTATACAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606151400_ymxl CAGGGTTTCTCTGTATAGCCCTGGCTCTCCTG(+AAAAAA)GTACCAAGGCAACTCTTATAAGGACAACATT NGG 10 8 17 8 24 0 100 5 10