Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606152633_fo8k TACTGCTACACAGCTTATTGGGTGTCAGTATG(+AAAAAA)GATCCGGAACTCGAGCTTCCAGACACGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606152631_vdx5 AGACTCGGCCAGAGACAGACGTTAGAGGACGG(+AAAAAA)GATCCAGTCCCTCTCCCACAGCAGGCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606152629_tmwg TAAGTGCTTTGCCTGCTGTGGGAGAGGGACTG(+AAAAAA)GATCCCGTCCTCTAACGTCTGTCTCTGGCCG NGG 10 8 17 8 24 0 100 5 10
    240606152628_7h2r GCAGAACCTAGGGCTTTGACCATGGCTTAGTG(+AAAAAA)GTACCATGCGAAGACACTTAGTGTGACAAGT NGG 10 8 17 8 24 0 100 5 10
    240606152627_1vi6 CTCAGACTTGTCACACTAAGTGTCTTCGCATG(+AAAAAA)GTACCACTAAGCCATGGTCAAAGCCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606152625_qi36 ATCTGCCCATTTCTGTCCCCTGGGGTACTGGG(+AAAAAA)GATCCAAACATAGTCCTCATGTCTGTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606152624_9gnz TGCTTACTGAACAGACATGAGGACTATGTTTG(+AAAAAA)GATCCCCAGTACCCCAGGGGACAGAAATGGG NGG 10 8 17 8 24 0 100 5 10
    240606152622_15qy AGTGTGCTTGCCTAGCATGTACAAAGCTCTGG(+AAAAAA)GATCCACCCCCAGCACCACTTAAGCCAGGCG NGG 10 8 17 8 24 0 100 5 10
    240606152620_pf42 CGCCACGCCTGGCTTAAGTGGTGCTGGGGGTG(+AAAAAA)GATCCCAGAGCTTTGTACATGCTAGGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240606152618_vkmg CTTTGCCAAGGCCCCCCGCTGTCCTCTACTAG(+AAAAAA)GATCCAACGGAAGCCTCTGCTCCCCCCGCCT NGG 10 8 17 8 24 0 100 5 10