Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606151049_itd9 CTCTTTGTAGCTGAGGGTGACTTTAAACTTCG(+AAAAAA)GATCCTCCTGCTTACCCACCCCCAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606151047_kj5a AATCCCAGCACTTGGGGGTGGGTAAGCAGGAG(+AAAAAA)GATCCGAAGTTTAAAGTCACCCTCAGCTACA NGG 10 8 17 8 24 0 100 5 10
    240606151046_8wmw TGGGTAGAGATTTGGTCCAGCGCATTCTCATG(+AAAAAA)GATCCTTCTCATCTTTTCCCAGGAACACTCG NGG 10 8 17 8 24 0 100 5 10
    240606151045_7bdk GGGAGCGAGTGTTCCTGGGAAAAGATGAGAAG(+AAAAAA)GATCCATGAGAATGCGCTGGACCAAATCTCT NGG 10 8 17 8 24 0 100 5 10
    240606151043_4yll GCTGTTTTCAAACACACCAGAAGAGGGCATAG(+AAAAAA)GATCCCATTACAGAGGGTTGTGAGTCACGGT NGG 10 8 17 8 24 0 100 5 10
    240606151042_djst ACCACACCGTGACTCACAACCCTCTGTAATGG(+AAAAAA)GATCCTATGCCCTCTTCTGGTGTGTTTGAAA NGG 10 8 17 8 24 0 100 5 10
    240606151040_l8m7 AAAGCAGTGGTTTGTACACACAGTACTGAAAG(+AAAAAA)GTACCCGAGGAGTCGCTACTCACAGTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606151038_6kto ACATATTTAAACTGTGAGTAGCGACTCCTCGG(+AAAAAA)GTACCTTTCAGTACTGTGTGTACAAACCACT NGG 10 8 17 8 24 0 100 5 10
    240606151036_ev6k CTGACAGACAGCCAAAATGGGGCAGGTTCCAG(+AAAAAA)GATCCTGATTGGTCGAGTGCTGGAGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606151035_2amn GGCTCTGCCTCCTCCAGCACTCGACCAATCAG(+AAAAAA)GATCCTGGAACCTGCCCCATTTTGGCTGTCT NGG 10 8 17 8 24 0 100 5 10