Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150543_im6w CATAGAAACATGAAGCTCCCACGCAAGGCGTG(+AAAAAA)GATCCAAACAAAGCACAGACAACACTGAACG NGG 10 8 17 8 24 0 100 5 10
    240606150542_u0u7 CTCCCCGTTCAGTGTTGTCTGTGCTTTGTTTG(+AAAAAA)GATCCACGCCTTGCGTGGGAGCTTCATGTTT NGG 10 8 17 8 24 0 100 5 10
    240606150540_hjun AGGACTGGCACAAGTTAATGGGGAAGAGGAAG(+AAAAAA)GTACCGCAGAACGGAGCTAATCCGTCACTGT NGG 10 8 17 8 24 0 100 5 10
    240606150539_2i40 AGGACACAGTGACGGATTAGCTCCGTTCTGCG(+AAAAAA)GTACCTTCCTCTTCCCCATTAACTTGTGCCA NGG 10 8 17 8 24 0 100 5 10
    240606150537_71fe GTGTGCTGGTCCAGCAGACAGCAGGACCAGAG(+AAAAAA)GTACCCGGCGACTCTTGGCAAACACCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606150535_cr9d GCCCAAGAAAGGTGTTTGCCAAGAGTCGCCGG(+AAAAAA)GTACCTCTGGTCCTGCTGTCTGCTGGACCAG NGG 10 8 17 8 24 0 100 5 10
    240606150533_xkk6 AGCAGAGTTTTAAAACCATCTCAGCCACCTCG(+AAAAAA)GATCCCAGAGAAGGACGACACTGAGTTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606150531_s4lt GGGGCTCAAAACTCAGTGTCGTCCTTCTCTGG(+AAAAAA)GATCCGAGGTGGCTGAGATGGTTTTAAAACT NGG 10 8 17 8 24 0 100 5 10
    240606150530_41mw CTGAAGTCTAGAAGCGTTGTCTCTCATCTGTG(+AAAAAA)GTACCCTCCCTGCGGTTCTCCAGTACCACTC NGG 10 8 17 8 24 0 100 5 10
    240606150529_jztd AAACTGAGTGGTACTGGAGAACCGCAGGGAGG(+AAAAAA)GTACCACAGATGAGAGACAACGCTTCTAGAC NGG 10 8 17 8 24 0 100 5 10