Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606150424_6ny1 GAACACAAGGAAACTCAGTCATGCTCAGAAAG(+AAAAAA)GATCCAGCTAAGTGAGCAGGTGGACTCCAAG NGG 10 8 17 8 24 0 100 5 10
    240606150423_lny3 TGAGCCTTGGAGTCCACCTGCTCACTTAGCTG(+AAAAAA)GATCCTTTCTGAGCATGACTGAGTTTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606150421_g9tz GCTGTTTTACAGCAGTGTGCCAAGAGATGTGG(+AAAAAA)GATCCACAGAGGGATTTTCTAACCTGGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606150420_c5qs GCCCCTGCTCCAGGTTAGAAAATCCCTCTGTG(+AAAAAA)GATCCCACATCTCTTGGCACACTGCTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240606150418_hy4m GAAAGGACAGGGTGTTCAGCACGTGTAGTTGG(+AAAAAA)GTACCTGGCCTAGAGGATACCCCTGAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240606150416_bmup CTAAGAAGCTTCAGGGGTATCCTCTAGGCCAG(+AAAAAA)GTACCCAACTACACGTGCTGAACACCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606150414_0bf9 GTAGGCCTGGCTAGCTAGCCAGTTTGCACTGG(+AAAAAA)GTACCCAGACTGGAATGGCAGGTGAGCCACC NGG 10 8 17 8 24 0 100 5 10
    240606150413_65os AACCAGGTGGCTCACCTGCCATTCCAGTCTGG(+AAAAAA)GTACCCAGTGCAAACTGGCTAGCTAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606150411_xrmh GCTGGCAGGCCCTGAAGCACAAGTTCAGGCCG(+AAAAAA)GATCCGGGCATCAGGACTTCGTGTTCATACA NGG 10 8 17 8 24 0 100 5 10
    240606150409_g4dy GTCCATGTATGAACACGAAGTCCTGATGCCCG(+AAAAAA)GATCCGGCCTGAACTTGTGCTTCAGGGCCTG NGG 10 8 17 8 24 0 100 5 10